Project acronym 3D2DPrint
Project 3D Printing of Novel 2D Nanomaterials: Adding Advanced 2D Functionalities to Revolutionary Tailored 3D Manufacturing
Researcher (PI) Valeria Nicolosi
Host Institution (HI) THE PROVOST, FELLOWS, FOUNDATION SCHOLARS & THE OTHER MEMBERS OF BOARD OF THE COLLEGE OF THE HOLY & UNDIVIDED TRINITY OF QUEEN ELIZABETH NEAR DUBLIN
Call Details Consolidator Grant (CoG), PE8, ERC-2015-CoG
Summary My vision is to establish, within the framework of an ERC CoG, a multidisciplinary group which will work in concert towards pioneering the integration of novel 2-Dimensional nanomaterials with novel additive fabrication techniques to develop a unique class of energy storage devices.
Batteries and supercapacitors are two very complementary types of energy storage devices. Batteries store much higher energy densities; supercapacitors, on the other hand, hold one tenth of the electricity per unit of volume or weight as compared to batteries but can achieve much higher power densities. Technology is currently striving to improve the power density of batteries and the energy density of supercapacitors. To do so it is imperative to develop new materials, chemistries and manufacturing strategies.
3D2DPrint aims to develop micro-energy devices (both supercapacitors and batteries), technologies particularly relevant in the context of the emergent industry of micro-electro-mechanical systems and constantly downsized electronics. We plan to use novel two-dimensional (2D) nanomaterials obtained by liquid-phase exfoliation. This method offers a new, economic and easy way to prepare ink of a variety of 2D systems, allowing to produce wide device performance window through elegant and simple constituent control at the point of fabrication. 3D2DPrint will use our expertise and know-how to allow development of advanced AM methods to integrate dissimilar nanomaterial blends and/or “hybrids” into fully embedded 3D printed energy storage devices, with the ultimate objective to realise a range of products that contain the above described nanomaterials subcomponent devices, electrical connections and traditional micro-fabricated subcomponents (if needed) ideally using a single tool.
Summary
My vision is to establish, within the framework of an ERC CoG, a multidisciplinary group which will work in concert towards pioneering the integration of novel 2-Dimensional nanomaterials with novel additive fabrication techniques to develop a unique class of energy storage devices.
Batteries and supercapacitors are two very complementary types of energy storage devices. Batteries store much higher energy densities; supercapacitors, on the other hand, hold one tenth of the electricity per unit of volume or weight as compared to batteries but can achieve much higher power densities. Technology is currently striving to improve the power density of batteries and the energy density of supercapacitors. To do so it is imperative to develop new materials, chemistries and manufacturing strategies.
3D2DPrint aims to develop micro-energy devices (both supercapacitors and batteries), technologies particularly relevant in the context of the emergent industry of micro-electro-mechanical systems and constantly downsized electronics. We plan to use novel two-dimensional (2D) nanomaterials obtained by liquid-phase exfoliation. This method offers a new, economic and easy way to prepare ink of a variety of 2D systems, allowing to produce wide device performance window through elegant and simple constituent control at the point of fabrication. 3D2DPrint will use our expertise and know-how to allow development of advanced AM methods to integrate dissimilar nanomaterial blends and/or “hybrids” into fully embedded 3D printed energy storage devices, with the ultimate objective to realise a range of products that contain the above described nanomaterials subcomponent devices, electrical connections and traditional micro-fabricated subcomponents (if needed) ideally using a single tool.
Max ERC Funding
2 499 942 €
Duration
Start date: 2016-10-01, End date: 2021-09-30
Project acronym 3DAddChip
Project Additive manufacturing of 2D nanomaterials for on-chip technologies
Researcher (PI) Cecilia Mattevi
Host Institution (HI) IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
Call Details Consolidator Grant (CoG), PE8, ERC-2018-COG
Summary The realization of “the internet of things” is inevitably constrained at the level of miniaturization that can be achieved in the electronic devices. A variety of technologies are now going through a process of miniaturization from micro-electromechanical systems (MEMS) to biomedical sensors, and actuators. The ultimate goal is to combine several components in an individual multifunctional platform, realizing on-chip technology. Devices have to be constrained to small footprints and exhibit high performance. Thus, the miniaturization process requires the introduction of new manufacturing processes to fabricate devices in the 3D space over small areas. 3D printing via robocasting is emerging as a new manufacturing technique, which allows shaping virtually any materials from polymers to ceramic and metals into complex architectures.
The goal of this research is to establish a 3D printing paradigm to produce miniaturized complex shape devices with diversified functions for on-chip technologies adaptable to “smart environment” such as flexible substrates, smart textiles and biomedical sensors. The elementary building blocks of the devices will be two-dimensional nanomaterials, which present unique optical, electrical, chemical and mechanical properties. The synergistic combination of the intrinsic characteristics of the 2D nanomaterials and the specific 3D architecture will enable advanced performance of the 3D printed objects. This research programme will demonstrate 3D miniaturized energy storage and energy conversion units fabricated with inks produced using a pilot plant. These units are essential components of any on-chip platform as they ensure energy autonomy via self-powering. Ultimately, this research will initiate new technologies based on miniaturized 3D devices.
Summary
The realization of “the internet of things” is inevitably constrained at the level of miniaturization that can be achieved in the electronic devices. A variety of technologies are now going through a process of miniaturization from micro-electromechanical systems (MEMS) to biomedical sensors, and actuators. The ultimate goal is to combine several components in an individual multifunctional platform, realizing on-chip technology. Devices have to be constrained to small footprints and exhibit high performance. Thus, the miniaturization process requires the introduction of new manufacturing processes to fabricate devices in the 3D space over small areas. 3D printing via robocasting is emerging as a new manufacturing technique, which allows shaping virtually any materials from polymers to ceramic and metals into complex architectures.
The goal of this research is to establish a 3D printing paradigm to produce miniaturized complex shape devices with diversified functions for on-chip technologies adaptable to “smart environment” such as flexible substrates, smart textiles and biomedical sensors. The elementary building blocks of the devices will be two-dimensional nanomaterials, which present unique optical, electrical, chemical and mechanical properties. The synergistic combination of the intrinsic characteristics of the 2D nanomaterials and the specific 3D architecture will enable advanced performance of the 3D printed objects. This research programme will demonstrate 3D miniaturized energy storage and energy conversion units fabricated with inks produced using a pilot plant. These units are essential components of any on-chip platform as they ensure energy autonomy via self-powering. Ultimately, this research will initiate new technologies based on miniaturized 3D devices.
Max ERC Funding
1 999 968 €
Duration
Start date: 2019-09-01, End date: 2024-08-31
Project acronym 3DPROTEINPUZZLES
Project Shape-directed protein assembly design
Researcher (PI) Lars Ingemar ANDRÉ
Host Institution (HI) LUNDS UNIVERSITET
Call Details Consolidator Grant (CoG), LS9, ERC-2017-COG
Summary Large protein complexes carry out some of the most complex functions in biology. Such structures are often assembled spontaneously from individual components through the process of self-assembly. If self-assembled protein complexes could be engineered from first principle it would enable a wide range of applications in biomedicine, nanotechnology and materials science. Recently, approaches to rationally design proteins to self-assembly into predefined structures have emerged. The highlight of this work is the design of protein cages that may be engineered into protein containers. However, current approaches for self-assembly design does not result in the assemblies with the required structural complexity to encode many of the sophisticated functions found in nature. To move forward, we have to learn how to engineer protein subunits with more than one designed interface that can assemble into tightly interacting complexes. In this proposal we propose a new protein design paradigm, shape directed protein design, in order to address shortcomings of the current methodology. The proposed method combines geometric shape matching and computational protein design. Using this approach we will de novo design assemblies with a wide variety of structural states, including protein complexes with cyclic and dihedral symmetry as well as icosahedral protein capsids built from novel protein building blocks. To enable these two design challenges we also develop a high-throughput assay to measure assembly stability in vivo that builds on a three-color fluorescent assay. This method will not only facilitate the screening of orders of magnitude more design constructs, but also enable the application of directed evolution to experimentally improve stable and assembly properties of designed containers as well as other designed assemblies.
Summary
Large protein complexes carry out some of the most complex functions in biology. Such structures are often assembled spontaneously from individual components through the process of self-assembly. If self-assembled protein complexes could be engineered from first principle it would enable a wide range of applications in biomedicine, nanotechnology and materials science. Recently, approaches to rationally design proteins to self-assembly into predefined structures have emerged. The highlight of this work is the design of protein cages that may be engineered into protein containers. However, current approaches for self-assembly design does not result in the assemblies with the required structural complexity to encode many of the sophisticated functions found in nature. To move forward, we have to learn how to engineer protein subunits with more than one designed interface that can assemble into tightly interacting complexes. In this proposal we propose a new protein design paradigm, shape directed protein design, in order to address shortcomings of the current methodology. The proposed method combines geometric shape matching and computational protein design. Using this approach we will de novo design assemblies with a wide variety of structural states, including protein complexes with cyclic and dihedral symmetry as well as icosahedral protein capsids built from novel protein building blocks. To enable these two design challenges we also develop a high-throughput assay to measure assembly stability in vivo that builds on a three-color fluorescent assay. This method will not only facilitate the screening of orders of magnitude more design constructs, but also enable the application of directed evolution to experimentally improve stable and assembly properties of designed containers as well as other designed assemblies.
Max ERC Funding
2 325 292 €
Duration
Start date: 2018-06-01, End date: 2023-05-31
Project acronym ADHESWITCHES
Project Adhesion switches in cancer and development: from in vivo to synthetic biology
Researcher (PI) Mari Johanna Ivaska
Host Institution (HI) TURUN YLIOPISTO
Call Details Consolidator Grant (CoG), LS3, ERC-2013-CoG
Summary Integrins are transmembrane cell adhesion receptors controlling cell proliferation and migration. Our objective is to gain fundamentally novel mechanistic insight into the emerging new roles of integrins in cancer and to generate a road map of integrin dependent pathways critical in mammary gland development and integrin signalling thus opening new targets for therapeutic interventions. We will combine an in vivo based translational approach with cell and molecular biological studies aiming to identify entirely novel concepts in integrin function using cutting edge techniques and synthetic-biology tools.
The specific objectives are:
1) Integrin inactivation in branching morphogenesis and cancer invasion. Integrins regulate mammary gland development and cancer invasion but the role of integrin inactivating proteins in these processes is currently completely unknown. We will investigate this using genetically modified mice, ex-vivo organoid models and human tissues with the aim to identify beneficial combinational treatments against cancer invasion.
2) Endosomal adhesomes – cross-talk between integrin activity and integrin “inside-in signaling”. We hypothesize that endocytosed active integrins engage in specialized endosomal signaling that governs cell survival especially in cancer. RNAi cell arrays, super-resolution STED imaging and endosomal proteomics will be used to investigate integrin signaling in endosomes.
3) Spatio-temporal co-ordination of adhesion and endocytosis. Several cytosolic proteins compete for integrin binding to regulate activation, endocytosis and recycling. Photoactivatable protein-traps and predefined matrix micropatterns will be employed to mechanistically dissect the spatio-temporal dynamics and hierarchy of their recruitment.
We will employ innovative and unconventional techniques to address three major unanswered questions in the field and significantly advance our understanding of integrin function in development and cancer.
Summary
Integrins are transmembrane cell adhesion receptors controlling cell proliferation and migration. Our objective is to gain fundamentally novel mechanistic insight into the emerging new roles of integrins in cancer and to generate a road map of integrin dependent pathways critical in mammary gland development and integrin signalling thus opening new targets for therapeutic interventions. We will combine an in vivo based translational approach with cell and molecular biological studies aiming to identify entirely novel concepts in integrin function using cutting edge techniques and synthetic-biology tools.
The specific objectives are:
1) Integrin inactivation in branching morphogenesis and cancer invasion. Integrins regulate mammary gland development and cancer invasion but the role of integrin inactivating proteins in these processes is currently completely unknown. We will investigate this using genetically modified mice, ex-vivo organoid models and human tissues with the aim to identify beneficial combinational treatments against cancer invasion.
2) Endosomal adhesomes – cross-talk between integrin activity and integrin “inside-in signaling”. We hypothesize that endocytosed active integrins engage in specialized endosomal signaling that governs cell survival especially in cancer. RNAi cell arrays, super-resolution STED imaging and endosomal proteomics will be used to investigate integrin signaling in endosomes.
3) Spatio-temporal co-ordination of adhesion and endocytosis. Several cytosolic proteins compete for integrin binding to regulate activation, endocytosis and recycling. Photoactivatable protein-traps and predefined matrix micropatterns will be employed to mechanistically dissect the spatio-temporal dynamics and hierarchy of their recruitment.
We will employ innovative and unconventional techniques to address three major unanswered questions in the field and significantly advance our understanding of integrin function in development and cancer.
Max ERC Funding
1 887 910 €
Duration
Start date: 2014-05-01, End date: 2019-04-30
Project acronym AdLibYeast
Project Synthetic platforms for ad libitum remodelling of yeast central metabolism
Researcher (PI) Pascale Andrée Simone Lapujade Daran
Host Institution (HI) TECHNISCHE UNIVERSITEIT DELFT
Call Details Consolidator Grant (CoG), LS9, ERC-2014-CoG
Summary Replacement of petrochemistry by bio-based processes is key to sustainable development and requires microbes equipped with novel-to-nature capabilities. The efficiency of such engineered microbes strongly depends on their native metabolic networks. However, aeons of evolution have optimized these networks for fitness in nature rather than for industrial performance. As a result, central metabolic networks are complex and encoded by mosaic microbial genomes in which genes, irrespective of their function, are scattered over the genome and chromosomes. This absence of a modular organization tremendously restricts genetic accessibility and presents a major hurdle for fundamental understanding and rational engineering of central metabolism. To conquer this limitation, I introduce the concept of ‘pathway swapping’, which will enable experimenters to remodel the core machinery of microbes at will.
Using the yeast Saccharomyces cerevisiae, an industrial biotechnology work horse and model eukaryotic cell, I propose to design and construct a microbial chassis in which all genes encoding enzymes in central carbon metabolism are relocated to a specialized synthetic chromosome, from which they can be easily swapped by any – homologous or heterologous – synthetic pathway. This challenging and innovative project paves the way for a modular approach to engineering of central metabolism.
Beyond providing a ground-breaking enabling technology, the ultimate goal of the pathway swapping technology is to address hitherto unanswered fundamental questions. Access to a sheer endless variety of configurations of central metabolism offers unique, new possibilities to study the fundamental design of metabolic pathways, the constraints that have shaped them and unifying principles for their structure and regulation. Moreover, this technology enables fast, combinatorial optimization studies on central metabolism to optimize its performance in biotechnological purposes.
Summary
Replacement of petrochemistry by bio-based processes is key to sustainable development and requires microbes equipped with novel-to-nature capabilities. The efficiency of such engineered microbes strongly depends on their native metabolic networks. However, aeons of evolution have optimized these networks for fitness in nature rather than for industrial performance. As a result, central metabolic networks are complex and encoded by mosaic microbial genomes in which genes, irrespective of their function, are scattered over the genome and chromosomes. This absence of a modular organization tremendously restricts genetic accessibility and presents a major hurdle for fundamental understanding and rational engineering of central metabolism. To conquer this limitation, I introduce the concept of ‘pathway swapping’, which will enable experimenters to remodel the core machinery of microbes at will.
Using the yeast Saccharomyces cerevisiae, an industrial biotechnology work horse and model eukaryotic cell, I propose to design and construct a microbial chassis in which all genes encoding enzymes in central carbon metabolism are relocated to a specialized synthetic chromosome, from which they can be easily swapped by any – homologous or heterologous – synthetic pathway. This challenging and innovative project paves the way for a modular approach to engineering of central metabolism.
Beyond providing a ground-breaking enabling technology, the ultimate goal of the pathway swapping technology is to address hitherto unanswered fundamental questions. Access to a sheer endless variety of configurations of central metabolism offers unique, new possibilities to study the fundamental design of metabolic pathways, the constraints that have shaped them and unifying principles for their structure and regulation. Moreover, this technology enables fast, combinatorial optimization studies on central metabolism to optimize its performance in biotechnological purposes.
Max ERC Funding
2 149 718 €
Duration
Start date: 2015-09-01, End date: 2020-08-31
Project acronym AFIRMATIVE
Project Acoustic-Flow Interaction Models for Advancing Thermoacoustic Instability prediction in Very low Emission combustors
Researcher (PI) Aimee MORGANS
Host Institution (HI) IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
Call Details Consolidator Grant (CoG), PE8, ERC-2017-COG
Summary Gas turbines are an essential ingredient in the long-term energy and aviation mix. They are flexible, offer fast start-up and the ability to burn renewable-generated fuels. However, they generate NOx emissions, which cause air pollution and damage human health, and reducing these is an air quality imperative. A major hurdle to this is that lean premixed combustion, essential for further NOx emission reductions, is highly susceptible to thermoacoustic instability. This is caused by a two-way coupling between unsteady combustion and acoustic waves, and the resulting large pressure oscillations can cause severe mechanical damage. Computational methods for predicting thermoacoustic instability, fast and accurate enough to be used as part of the industrial design process, are urgently needed.
The only computational methods with the prospect of being fast enough are those based on coupled treatment of the acoustic waves and unsteady combustion. These exploit the amenity of the acoustic waves to analytical modelling, allowing costly simulations to be directed only at the more complex flame. They show real promise: my group recently demonstrated the first accurate coupled predictions for lab-scale combustors. The method does not yet extend to industrial combustors, the more complex flow-fields in these rendering current acoustic models overly-simplistic. I propose to comprehensively overhaul acoustic models across the entirety of the combustor, accounting for real and important acoustic-flow interactions. These new models will offer the breakthrough prospect of extending efficient, accurate predictive capability to industrial combustors, which has a real chance of facilitating future, instability free, very low NOx gas turbines.
Summary
Gas turbines are an essential ingredient in the long-term energy and aviation mix. They are flexible, offer fast start-up and the ability to burn renewable-generated fuels. However, they generate NOx emissions, which cause air pollution and damage human health, and reducing these is an air quality imperative. A major hurdle to this is that lean premixed combustion, essential for further NOx emission reductions, is highly susceptible to thermoacoustic instability. This is caused by a two-way coupling between unsteady combustion and acoustic waves, and the resulting large pressure oscillations can cause severe mechanical damage. Computational methods for predicting thermoacoustic instability, fast and accurate enough to be used as part of the industrial design process, are urgently needed.
The only computational methods with the prospect of being fast enough are those based on coupled treatment of the acoustic waves and unsteady combustion. These exploit the amenity of the acoustic waves to analytical modelling, allowing costly simulations to be directed only at the more complex flame. They show real promise: my group recently demonstrated the first accurate coupled predictions for lab-scale combustors. The method does not yet extend to industrial combustors, the more complex flow-fields in these rendering current acoustic models overly-simplistic. I propose to comprehensively overhaul acoustic models across the entirety of the combustor, accounting for real and important acoustic-flow interactions. These new models will offer the breakthrough prospect of extending efficient, accurate predictive capability to industrial combustors, which has a real chance of facilitating future, instability free, very low NOx gas turbines.
Max ERC Funding
1 985 288 €
Duration
Start date: 2018-06-01, End date: 2023-05-31
Project acronym AMADEUS
Project Advancing CO2 Capture Materials by Atomic Scale Design: the Quest for Understanding
Researcher (PI) Christoph Rüdiger MÜLLER
Host Institution (HI) EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Call Details Consolidator Grant (CoG), PE8, ERC-2018-COG
Summary Carbon dioxide capture and storage is a technology to mitigate climate change by removing CO2 from flue gas streams or the atmosphere and storing it in geological formations. While CO2 removal from natural gas by amine scrubbing is implemented on the large scale, the cost of such process is currently prohibitively expensive. Inexpensive alkali earth metal oxides (MgO and CaO) feature high theoretical CO2 uptakes, but suffer from poor cyclic stability and slow kinetics. Yet, the key objective of recent research on alkali earth metal oxide based CO2 sorbents has been the processing of inexpensive, naturally occurring CO2 sorbents, notably limestone and dolomite, to stabilize their modest CO2 uptake and to establish re-activation methods through engineering approaches. While this research demonstrated a landmark Megawatt (MW) scale viability of the process, our fundamental understanding of the underlying CO2 capture, regeneration and deactivation pathways did not improve. The latter knowledge is, however, vital for the rational design of improved, yet practical CaO and MgO sorbents. Hence this proposal is concerned with obtaining an understanding of the underlying mechanisms that control the ability of an alkali metal oxide to capture a large quantity of CO2 with a high rate, to regenerate and to operate with high cyclic stability. Achieving these aims relies on the ability to fabricate model structures and to characterize in great detail their surface chemistry, morphology, chemical composition and changes therein under reactive conditions. This makes the development of operando and in situ characterization tools an essential prerequisite. Advances in these areas shall allow achieving the overall goal of this project, viz. to formulate a roadmap to fabricate improved CO2 sorbents through their precisely engineered structure, composition and morphology.
Summary
Carbon dioxide capture and storage is a technology to mitigate climate change by removing CO2 from flue gas streams or the atmosphere and storing it in geological formations. While CO2 removal from natural gas by amine scrubbing is implemented on the large scale, the cost of such process is currently prohibitively expensive. Inexpensive alkali earth metal oxides (MgO and CaO) feature high theoretical CO2 uptakes, but suffer from poor cyclic stability and slow kinetics. Yet, the key objective of recent research on alkali earth metal oxide based CO2 sorbents has been the processing of inexpensive, naturally occurring CO2 sorbents, notably limestone and dolomite, to stabilize their modest CO2 uptake and to establish re-activation methods through engineering approaches. While this research demonstrated a landmark Megawatt (MW) scale viability of the process, our fundamental understanding of the underlying CO2 capture, regeneration and deactivation pathways did not improve. The latter knowledge is, however, vital for the rational design of improved, yet practical CaO and MgO sorbents. Hence this proposal is concerned with obtaining an understanding of the underlying mechanisms that control the ability of an alkali metal oxide to capture a large quantity of CO2 with a high rate, to regenerate and to operate with high cyclic stability. Achieving these aims relies on the ability to fabricate model structures and to characterize in great detail their surface chemistry, morphology, chemical composition and changes therein under reactive conditions. This makes the development of operando and in situ characterization tools an essential prerequisite. Advances in these areas shall allow achieving the overall goal of this project, viz. to formulate a roadmap to fabricate improved CO2 sorbents through their precisely engineered structure, composition and morphology.
Max ERC Funding
1 994 900 €
Duration
Start date: 2019-06-01, End date: 2024-05-31
Project acronym AnCon
Project A Comparative Anthropology of Conscience, Ethics and Human Rights
Researcher (PI) Tobias William Kelly
Host Institution (HI) THE UNIVERSITY OF EDINBURGH
Call Details Consolidator Grant (CoG), SH2, ERC-2014-CoG
Summary This project is a comparative anthropology of conscience, ethics and human rights. Numerous international human rights documents formally declare their commitment to protect freedom of conscience. But, what is conscience and how do we know it when we see it? How do we distinguish it from self-interest or fanaticism? And what happens when the concept, often associated with a distinct Christian or liberal history, travels across cultural boundaries? The project will examine the cultural conditions under which claims to conscience are made possible, and the types of claims that are most persuasive when doing so. The project addresses these issues through the comparative analysis of three case studies: British pacifists, Sri Lankan activists, and Soviet dissidents. These case studies have been carefully chosen to provide globally significant, but contrasting examples of contests over the implications of claims to conscience. If claims of conscience are often associated with a specifically liberal and Christian tradition, mid-twentieth century Britain can be said to stand at the centre of that tradition. Sri Lanka represents a particularly fraught post-colonial South Asian counterpoint, wracked by nationalist violence, and influenced by ethical traditions associated with forms of Hinduism and Buddhism. Soviet Russia represents a further contrast, a totalitarian regime, where atheism was the dominant ethical language. Finally, the project will return specifically to international human rights institutions, examining the history of the category of conscience in the UN human rights system. This project will be ground breaking, employing novel methods and analytical insights, in order to producing the first comparative analysis of the cultural and political salience of claims of conscience. In doing so, the research aims to transform our understandings of the limits and potentials of attempts to protect freedom of conscience.
Summary
This project is a comparative anthropology of conscience, ethics and human rights. Numerous international human rights documents formally declare their commitment to protect freedom of conscience. But, what is conscience and how do we know it when we see it? How do we distinguish it from self-interest or fanaticism? And what happens when the concept, often associated with a distinct Christian or liberal history, travels across cultural boundaries? The project will examine the cultural conditions under which claims to conscience are made possible, and the types of claims that are most persuasive when doing so. The project addresses these issues through the comparative analysis of three case studies: British pacifists, Sri Lankan activists, and Soviet dissidents. These case studies have been carefully chosen to provide globally significant, but contrasting examples of contests over the implications of claims to conscience. If claims of conscience are often associated with a specifically liberal and Christian tradition, mid-twentieth century Britain can be said to stand at the centre of that tradition. Sri Lanka represents a particularly fraught post-colonial South Asian counterpoint, wracked by nationalist violence, and influenced by ethical traditions associated with forms of Hinduism and Buddhism. Soviet Russia represents a further contrast, a totalitarian regime, where atheism was the dominant ethical language. Finally, the project will return specifically to international human rights institutions, examining the history of the category of conscience in the UN human rights system. This project will be ground breaking, employing novel methods and analytical insights, in order to producing the first comparative analysis of the cultural and political salience of claims of conscience. In doing so, the research aims to transform our understandings of the limits and potentials of attempts to protect freedom of conscience.
Max ERC Funding
1 457 869 €
Duration
Start date: 2015-08-01, End date: 2020-07-31
Project acronym APOLOGY
Project Political Apologies across Cultures
Researcher (PI) Juliëtte Schaafsma
Host Institution (HI) STICHTING KATHOLIEKE UNIVERSITEIT BRABANT
Call Details Consolidator Grant (CoG), SH2, ERC-2015-CoG
Summary In the past decades, there has been a considerable rise in the number of apologies offered by states for injustices and human rights violations. Among transitional justice scholars, there is significant debate about how useful such apologies are. Whereas some have applauded these gestures as an important step in peacemaking processes, others have argued that they may not fit in all cultures and may even be a risky tool for peacemaking. Unfortunately, theorizing and research in the field of transitional justice is still in its infancy and has not systematically addressed questions of cross-cultural variability yet. So, at present, we do not know whether political apologies are a universally viable way to restore justice and harmony. My project addresses this challenge. Using an innovative, interdisciplinary, and multi-method approach with in-depth interviews, (experimental) surveys, and content analyses of apologies, I analyze whether there are universals in how political apologies are valued, expressed, and interpreted or whether this varies as a function of cross-cultural differences in key values (collectivism and individualism) and norms (face and honor). Based on these findings, I build a theoretical framework that will fundamentally advance our understanding of the potential value and role of apologies in transitional justice processes. This project breaks new ground because it is the first to take the difficult step to collect cross-cultural data to examine whether key assumptions regarding political apologies hold across cultures. It is also the first in this area to use a multi-method approach, which makes it possible to take into account the complex reality of political apologies. Combining insights from transitional justice, cross-cultural psychology and anthropology, this project places theorizing on transitional justice on a much firmer footing and paves the way to more cross-culturally valid models to restore justice and promote reconciliation.
Summary
In the past decades, there has been a considerable rise in the number of apologies offered by states for injustices and human rights violations. Among transitional justice scholars, there is significant debate about how useful such apologies are. Whereas some have applauded these gestures as an important step in peacemaking processes, others have argued that they may not fit in all cultures and may even be a risky tool for peacemaking. Unfortunately, theorizing and research in the field of transitional justice is still in its infancy and has not systematically addressed questions of cross-cultural variability yet. So, at present, we do not know whether political apologies are a universally viable way to restore justice and harmony. My project addresses this challenge. Using an innovative, interdisciplinary, and multi-method approach with in-depth interviews, (experimental) surveys, and content analyses of apologies, I analyze whether there are universals in how political apologies are valued, expressed, and interpreted or whether this varies as a function of cross-cultural differences in key values (collectivism and individualism) and norms (face and honor). Based on these findings, I build a theoretical framework that will fundamentally advance our understanding of the potential value and role of apologies in transitional justice processes. This project breaks new ground because it is the first to take the difficult step to collect cross-cultural data to examine whether key assumptions regarding political apologies hold across cultures. It is also the first in this area to use a multi-method approach, which makes it possible to take into account the complex reality of political apologies. Combining insights from transitional justice, cross-cultural psychology and anthropology, this project places theorizing on transitional justice on a much firmer footing and paves the way to more cross-culturally valid models to restore justice and promote reconciliation.
Max ERC Funding
1 917 713 €
Duration
Start date: 2016-09-01, End date: 2021-08-31
Project acronym APOSITE
Project Apoptotic foci: composition, structure and dynamics
Researcher (PI) Ana GARCIA SAEZ
Host Institution (HI) EBERHARD KARLS UNIVERSITAET TUEBINGEN
Call Details Consolidator Grant (CoG), LS3, ERC-2018-COG
Summary Apoptotic cell death is essential for development, immune function or tissue homeostasis, and it is often deregulated in disease. Mitochondrial outer membrane permeabilization (MOMP) is central for apoptosis execution and plays a key role in its inflammatory outcome. Knowing the architecture of the macromolecular machineries mediating MOMP is crucial for understanding their function and for the clinical use of apoptosis.
Our recent work reveals that Bax and Bak dimers form distinct line, arc and ring assemblies at specific apoptotic foci to mediate MOMP. However, the molecular structure and mechanisms controlling the spatiotemporal formation and range of action of the apoptotic foci are missing. To address this fundamental gap in our knowledge, we aim to unravel the composition, dynamics and structure of apoptotic foci and to understand how they are integrated to orchestrate function. We will reach this goal by building on our expertise in cell death and cutting-edge imaging and by developing a new analytical pipeline to:
1) Identify the composition of apoptotic foci using in situ proximity-dependent labeling and extraction of near-native Bax/Bak membrane complexes coupled to mass spectrometry.
2) Define their contribution to apoptosis and its immunogenicity and establish their assembly dynamics to correlate it with apoptosis progression by live cell imaging.
3) Determine the stoichiometry and structural organization of the apoptotic foci by combining single molecule fluorescence and advanced electron microscopies.
This multidisciplinary approach offers high chances to solve the long-standing question of how Bax and Bak mediate MOMP. APOSITE will provide textbook knowledge of the mitochondrial contribution to cell death and inflammation. The implementation of this new analytical framework will open novel research avenues in membrane and organelle biology. Ultimately, understanding of Bax and Bak structure/function will help develop apoptosis modulators for medicine.
Summary
Apoptotic cell death is essential for development, immune function or tissue homeostasis, and it is often deregulated in disease. Mitochondrial outer membrane permeabilization (MOMP) is central for apoptosis execution and plays a key role in its inflammatory outcome. Knowing the architecture of the macromolecular machineries mediating MOMP is crucial for understanding their function and for the clinical use of apoptosis.
Our recent work reveals that Bax and Bak dimers form distinct line, arc and ring assemblies at specific apoptotic foci to mediate MOMP. However, the molecular structure and mechanisms controlling the spatiotemporal formation and range of action of the apoptotic foci are missing. To address this fundamental gap in our knowledge, we aim to unravel the composition, dynamics and structure of apoptotic foci and to understand how they are integrated to orchestrate function. We will reach this goal by building on our expertise in cell death and cutting-edge imaging and by developing a new analytical pipeline to:
1) Identify the composition of apoptotic foci using in situ proximity-dependent labeling and extraction of near-native Bax/Bak membrane complexes coupled to mass spectrometry.
2) Define their contribution to apoptosis and its immunogenicity and establish their assembly dynamics to correlate it with apoptosis progression by live cell imaging.
3) Determine the stoichiometry and structural organization of the apoptotic foci by combining single molecule fluorescence and advanced electron microscopies.
This multidisciplinary approach offers high chances to solve the long-standing question of how Bax and Bak mediate MOMP. APOSITE will provide textbook knowledge of the mitochondrial contribution to cell death and inflammation. The implementation of this new analytical framework will open novel research avenues in membrane and organelle biology. Ultimately, understanding of Bax and Bak structure/function will help develop apoptosis modulators for medicine.
Max ERC Funding
2 000 000 €
Duration
Start date: 2019-04-01, End date: 2024-03-31
