Project acronym eCAPE
Project New energy Consumer roles and smart technologies – Actors, Practices and Equality
Researcher (PI) Kirsten GRAM-HANSSEN
Host Institution (HI) AALBORG UNIVERSITET
Call Details Advanced Grant (AdG), SH2, ERC-2017-ADG
Summary The transition to a low-carbon society is vital and requires major changes in everyday life for European households, including new prosumer roles linking renewable energy production and household consumption by use of smart technologies. This implies major alterations in the materiality as well as the social organisation of everyday life. To guide this low-carbon transition, new theory development on the role of technological systems in everyday life is needed. Practice theories represent a strong approach in this; however, they have developed in opposition to understanding actors and structures as mutually interlinked. This means that major drivers, as well as consequences, for sustainable transition are being overlooked. This project will contribute with important new theory development to understand and promote a low-carbon transition as well as to ensure that this transition does not indirectly become a driver of gender and social inequality.
Three theoretical lines within theories of practice will be developed:
1. The importance of gender and social structures when studying household practices, including how these social structures influence formation of practices and how, in turn, social structures are formed by the development of practices.
2. The role of the ethical consumer in developing new practices, including how learning processes, media discourses and institutionalised knowledge influence formation of practices.
3. The inclusion of non-humans as carriers and performers of practices, rather than seeing the material arrangements only as the context for practices, especially when dealing with automated and internet connected technologies.
Quantitative and qualitative empirical research guided by these theoretical approaches will contribute with work on how future low-carbon living can be achieved and the theoretical developments will form an essential foundation for policy development towards a mandatory low-carbon transition.
Summary
The transition to a low-carbon society is vital and requires major changes in everyday life for European households, including new prosumer roles linking renewable energy production and household consumption by use of smart technologies. This implies major alterations in the materiality as well as the social organisation of everyday life. To guide this low-carbon transition, new theory development on the role of technological systems in everyday life is needed. Practice theories represent a strong approach in this; however, they have developed in opposition to understanding actors and structures as mutually interlinked. This means that major drivers, as well as consequences, for sustainable transition are being overlooked. This project will contribute with important new theory development to understand and promote a low-carbon transition as well as to ensure that this transition does not indirectly become a driver of gender and social inequality.
Three theoretical lines within theories of practice will be developed:
1. The importance of gender and social structures when studying household practices, including how these social structures influence formation of practices and how, in turn, social structures are formed by the development of practices.
2. The role of the ethical consumer in developing new practices, including how learning processes, media discourses and institutionalised knowledge influence formation of practices.
3. The inclusion of non-humans as carriers and performers of practices, rather than seeing the material arrangements only as the context for practices, especially when dealing with automated and internet connected technologies.
Quantitative and qualitative empirical research guided by these theoretical approaches will contribute with work on how future low-carbon living can be achieved and the theoretical developments will form an essential foundation for policy development towards a mandatory low-carbon transition.
Max ERC Funding
2 116 000 €
Duration
Start date: 2018-11-01, End date: 2023-10-31
Project acronym MaCChines
Project Molecular machines based on coiled-coil protein origami
Researcher (PI) Roman JERALA
Host Institution (HI) KEMIJSKI INSTITUT
Call Details Advanced Grant (AdG), LS9, ERC-2017-ADG
Summary Proteins are the most versatile and complex smart nanomaterials, forming molecular machines and performing numerous functions from structure building, recognition, catalysis to locomotion. Nature however explored only a tiny fraction of possible protein sequences and structures. Design of proteins with new, in nature unseen shapes and features, offers high rewards for medicine, technology and science. In 2013 my group pioneered the design of a new type of modular coiled-coil protein origami (CCPO) folds. This type of de novo designed proteins are defined by the sequence of coiled-coil (CC) dimer-forming modules that are concatenated by flexible linkers into a single polypeptide chain that self-assembles into a polyhedral cage based on pairwise CC interactions. This is in contrast to naturally evolved proteins where their fold is defined by a compact hydrophobic core. We recently demonstrated the robustness of this strategy by the largest de novo designed single chain protein, construction of tetrahedral, pyramid, trigonal prism and bipyramid cages that self-assemble in vivo.
This proposal builds on unique advantages of CCPOs and represents a new frontier of this branch of protein design science. I propose to introduce functional domains into selected positions of CCPO cages, implement new types of building modules that will enable regulated CCPO assembly and disassembly, test new strategies of caging and release of cargo molecules for targeted delivery, design knotted and crosslinked protein cages and introduce toehold displacement for the regulated structural rearrangement of CCPOs required for designed molecular machines, which will be demonstrated on protein nanotweezers. Technology for the positional combinatorial library-based single pot assembly of CCPO genes will provide high throughput of CCPO variants. Project will result in new methodology, understanding of potentials of CCPOs for designed molecular machines and in demonstration of different applications.
Summary
Proteins are the most versatile and complex smart nanomaterials, forming molecular machines and performing numerous functions from structure building, recognition, catalysis to locomotion. Nature however explored only a tiny fraction of possible protein sequences and structures. Design of proteins with new, in nature unseen shapes and features, offers high rewards for medicine, technology and science. In 2013 my group pioneered the design of a new type of modular coiled-coil protein origami (CCPO) folds. This type of de novo designed proteins are defined by the sequence of coiled-coil (CC) dimer-forming modules that are concatenated by flexible linkers into a single polypeptide chain that self-assembles into a polyhedral cage based on pairwise CC interactions. This is in contrast to naturally evolved proteins where their fold is defined by a compact hydrophobic core. We recently demonstrated the robustness of this strategy by the largest de novo designed single chain protein, construction of tetrahedral, pyramid, trigonal prism and bipyramid cages that self-assemble in vivo.
This proposal builds on unique advantages of CCPOs and represents a new frontier of this branch of protein design science. I propose to introduce functional domains into selected positions of CCPO cages, implement new types of building modules that will enable regulated CCPO assembly and disassembly, test new strategies of caging and release of cargo molecules for targeted delivery, design knotted and crosslinked protein cages and introduce toehold displacement for the regulated structural rearrangement of CCPOs required for designed molecular machines, which will be demonstrated on protein nanotweezers. Technology for the positional combinatorial library-based single pot assembly of CCPO genes will provide high throughput of CCPO variants. Project will result in new methodology, understanding of potentials of CCPOs for designed molecular machines and in demonstration of different applications.
Max ERC Funding
2 497 125 €
Duration
Start date: 2018-09-01, End date: 2023-08-31
Project acronym YEAST-TRANS
Project Deciphering the transport mechanisms of small xenobiotic molecules in synthetic yeast cell factories
Researcher (PI) Irina BORODINA
Host Institution (HI) DANMARKS TEKNISKE UNIVERSITET
Call Details Starting Grant (StG), LS9, ERC-2017-STG
Summary Industrial biotechnology employs synthetic cell factories to create bulk and fine chemicals and fuels from renewable resources, laying the basis for the future bio-based economy. The major part of the wanted bio-based chemicals are not native to the host cell, such as yeast, i.e. they are xenobiotic. Some xenobiotic compounds are readily secreted by synthetic cells, some are poorly secreted and some are not secreted at all, but how does this transport occur? Or why does it not occur? These fundamental questions remain to be answered and this will have great implications on industrial biotechnology, because improved secretion would bring down the production costs and enable the emergence of novel bio-based products.
YEAST-TRANS will fill in this knowledge gap by carrying out the first systematic genome-scale transporter study to uncover the transport mechanisms of small xenobiotic molecules by synthetic yeast cells and to apply this knowledge for engineering more efficient cell factories for bio-based production of fuels and chemicals.
Summary
Industrial biotechnology employs synthetic cell factories to create bulk and fine chemicals and fuels from renewable resources, laying the basis for the future bio-based economy. The major part of the wanted bio-based chemicals are not native to the host cell, such as yeast, i.e. they are xenobiotic. Some xenobiotic compounds are readily secreted by synthetic cells, some are poorly secreted and some are not secreted at all, but how does this transport occur? Or why does it not occur? These fundamental questions remain to be answered and this will have great implications on industrial biotechnology, because improved secretion would bring down the production costs and enable the emergence of novel bio-based products.
YEAST-TRANS will fill in this knowledge gap by carrying out the first systematic genome-scale transporter study to uncover the transport mechanisms of small xenobiotic molecules by synthetic yeast cells and to apply this knowledge for engineering more efficient cell factories for bio-based production of fuels and chemicals.
Max ERC Funding
1 423 358 €
Duration
Start date: 2017-12-01, End date: 2022-11-30
