Project acronym 0MSPIN
Project Spintronics based on relativistic phenomena in systems with zero magnetic moment
Researcher (PI) Tomá Jungwirth
Host Institution (HI) FYZIKALNI USTAV AV CR V.V.I
Call Details Advanced Grant (AdG), PE3, ERC-2010-AdG_20100224
Summary The 0MSPIN project consists of an extensive integrated theoretical, experimental and device development programme of research opening a radical new approach to spintronics. Spintronics has the potential to supersede existing storage and memory applications, and to provide alternatives to current CMOS technology. Ferromagnetic matels used in all current spintronics applications may make it impractical to realise the full potential of spintronics. Metals are unsuitable for transistor and information processing applications, for opto-electronics, or for high-density integration. The 0MSPIN project aims to remove the major road-block holding back the development of spintronics in a radical way: removing the ferromagnetic component from key active parts or from the whole of the spintronic devices. This approach is based on exploiting the combination of exchange and spin-orbit coupling phenomena and material systems with zero macroscopic moment. The goal of the 0MSPIN is to provide a new paradigm by which spintronics can enter the realms of conventional semiconductors in both fundamental condensed matter research and in information technologies. In the central part of the proposal, the research towards this goal is embedded within a materials science project whose aim is to introduce into physics and microelectronics an entirely new class of semiconductors. 0MSPIN seeks to exploit three classes of material systems: (1) Antiferromagnetic bi-metallic 3d-5d alloys (e.g. Mn2Au). (2) Antiferromagnetic I-II-V semiconductors (e.g. LiMnAs). (3) Non-magnetic spin-orbit coupled semiconductors with injected spin-polarized currents (e.g. 2D III-V structures). Proof of concept devices operating at high temperatures will be fabricated to show-case new functionalities offered by zero-moment systems for sensing and memory applications, information processing, and opto-electronics technologies.
Summary
The 0MSPIN project consists of an extensive integrated theoretical, experimental and device development programme of research opening a radical new approach to spintronics. Spintronics has the potential to supersede existing storage and memory applications, and to provide alternatives to current CMOS technology. Ferromagnetic matels used in all current spintronics applications may make it impractical to realise the full potential of spintronics. Metals are unsuitable for transistor and information processing applications, for opto-electronics, or for high-density integration. The 0MSPIN project aims to remove the major road-block holding back the development of spintronics in a radical way: removing the ferromagnetic component from key active parts or from the whole of the spintronic devices. This approach is based on exploiting the combination of exchange and spin-orbit coupling phenomena and material systems with zero macroscopic moment. The goal of the 0MSPIN is to provide a new paradigm by which spintronics can enter the realms of conventional semiconductors in both fundamental condensed matter research and in information technologies. In the central part of the proposal, the research towards this goal is embedded within a materials science project whose aim is to introduce into physics and microelectronics an entirely new class of semiconductors. 0MSPIN seeks to exploit three classes of material systems: (1) Antiferromagnetic bi-metallic 3d-5d alloys (e.g. Mn2Au). (2) Antiferromagnetic I-II-V semiconductors (e.g. LiMnAs). (3) Non-magnetic spin-orbit coupled semiconductors with injected spin-polarized currents (e.g. 2D III-V structures). Proof of concept devices operating at high temperatures will be fabricated to show-case new functionalities offered by zero-moment systems for sensing and memory applications, information processing, and opto-electronics technologies.
Max ERC Funding
1 938 000 €
Duration
Start date: 2011-06-01, End date: 2016-05-31
Project acronym 123STABLE
Project Towards Nanostructured Electrocatalysts with Superior Stability
Researcher (PI) Nejc HODNIK
Host Institution (HI) KEMIJSKI INSTITUT
Call Details Starting Grant (StG), PE4, ERC-2019-STG
Summary In the last decades, significant progress has been made on understanding and controlling solid/liquid electrochemical interfaces at atomic levels. As the principles guiding the activity of electrochemical reactions are quite well established (structure-activity relationships), the fundamentals of stability are still poorly understood (structure-stability relationships). 123STABLE proposes to employ (1) identical location, (2) online monitoring and (3) modeling of noble metals based nanoparticles changes with the state-of-the-art electron microscopy equipment and online dissolution and evolution analytics using electrochemical flow cell coupled to online mass spectrometers. Projects unique methodology approach with picogram sensitivity levels, in combination with sub-atomic scale microscopy insights and simulations, promises novel atomistic insights into the corrosion and reconstruction of noble metals in electrochemical environments. This unique approach is based on observations of the same nanoparticles before and after electrochemical treatment where weak and stable atomic features and events can be recognized, followed, understood and finally utilized. Upon (1) doping, (2) decoration and/or (3) other synthetic modification of nanoparticles like a change in size and shape further stabilization is envisioned. For instance, blockage of nanoparticle vulnerable defected sites like steps or kinks by more noble metal could stop or significantly slow down their degradation.
The 123STABLE project will feature platinum- and iridium-based nanostructures as a model system to introduce a unique “123” approach, as they still possess the best electrocatalytic properties for the future electrification of society through the Hydrogen economy. However, their electrochemical stability is still not sufficient. Coupled with the fact that their supply is hindered by extremely scarce, rare and uneven geological distribution, the increase in their stability is of immense importance.
Summary
In the last decades, significant progress has been made on understanding and controlling solid/liquid electrochemical interfaces at atomic levels. As the principles guiding the activity of electrochemical reactions are quite well established (structure-activity relationships), the fundamentals of stability are still poorly understood (structure-stability relationships). 123STABLE proposes to employ (1) identical location, (2) online monitoring and (3) modeling of noble metals based nanoparticles changes with the state-of-the-art electron microscopy equipment and online dissolution and evolution analytics using electrochemical flow cell coupled to online mass spectrometers. Projects unique methodology approach with picogram sensitivity levels, in combination with sub-atomic scale microscopy insights and simulations, promises novel atomistic insights into the corrosion and reconstruction of noble metals in electrochemical environments. This unique approach is based on observations of the same nanoparticles before and after electrochemical treatment where weak and stable atomic features and events can be recognized, followed, understood and finally utilized. Upon (1) doping, (2) decoration and/or (3) other synthetic modification of nanoparticles like a change in size and shape further stabilization is envisioned. For instance, blockage of nanoparticle vulnerable defected sites like steps or kinks by more noble metal could stop or significantly slow down their degradation.
The 123STABLE project will feature platinum- and iridium-based nanostructures as a model system to introduce a unique “123” approach, as they still possess the best electrocatalytic properties for the future electrification of society through the Hydrogen economy. However, their electrochemical stability is still not sufficient. Coupled with the fact that their supply is hindered by extremely scarce, rare and uneven geological distribution, the increase in their stability is of immense importance.
Max ERC Funding
1 496 750 €
Duration
Start date: 2020-01-01, End date: 2024-12-31
Project acronym 2D4D
Project Disruptive Digitalization for Decarbonization
Researcher (PI) Elena Verdolini
Host Institution (HI) UNIVERSITA DEGLI STUDI DI BRESCIA
Call Details Starting Grant (StG), SH2, ERC-2019-STG
Summary By 2040, all major sectors of the European economy will be deeply digitalized. By then, the EU aims at reducing greenhouse gas emissions by 60% with respect to 1990 levels. Digitalization will affect decarbonization efforts because of its impacts on energy demand, employment, competitiveness, trade patterns and its distributional, behavioural and ethical implications. Yet, the policy debates around these two transformations are largely disjoint.
The aim of the 2D4D project is ensure that the digital revolution acts as an enabler – and not as a barrier – for decarbonization. The project quantifies the decarbonization implications of three disruptive digitalization technologies in hard-to-decarbonize sectors: (1) Additive Manufacturing in industry, (2) Mobility-as-a-Service in transportation, and (3) Artificial Intelligence in buildings.
The first objective of 2D4D is to generate a one-of-a-kind data collection to investigate the technical and socio-economic dynamics of these technologies, and how they may affect decarbonization narratives and scenarios. This will be achieved through several data collection methods, including desk research, surveys and expert elicitations.
The second objective of 2D4D is to include digitalization dynamics in decarbonization narratives and pathways. On the one hand, this entails enhancing decarbonization narratives (specifically, the Shared Socio-economic Pathways) to describe digitalization dynamics. On the other hand, it requires improving the representation of sector-specific digitalization dynamics in Integrated Assessment Models, one of the main tools available to generate decarbonization pathways.
The third objective of 2D4D is to identify no-regret, robust policy portfolios. These will be designed to ensure that digitalization unfolds in an inclusive, climate-beneficial way, and that decarbonization policies capitalize on digital technologies to support the energy transition.
Summary
By 2040, all major sectors of the European economy will be deeply digitalized. By then, the EU aims at reducing greenhouse gas emissions by 60% with respect to 1990 levels. Digitalization will affect decarbonization efforts because of its impacts on energy demand, employment, competitiveness, trade patterns and its distributional, behavioural and ethical implications. Yet, the policy debates around these two transformations are largely disjoint.
The aim of the 2D4D project is ensure that the digital revolution acts as an enabler – and not as a barrier – for decarbonization. The project quantifies the decarbonization implications of three disruptive digitalization technologies in hard-to-decarbonize sectors: (1) Additive Manufacturing in industry, (2) Mobility-as-a-Service in transportation, and (3) Artificial Intelligence in buildings.
The first objective of 2D4D is to generate a one-of-a-kind data collection to investigate the technical and socio-economic dynamics of these technologies, and how they may affect decarbonization narratives and scenarios. This will be achieved through several data collection methods, including desk research, surveys and expert elicitations.
The second objective of 2D4D is to include digitalization dynamics in decarbonization narratives and pathways. On the one hand, this entails enhancing decarbonization narratives (specifically, the Shared Socio-economic Pathways) to describe digitalization dynamics. On the other hand, it requires improving the representation of sector-specific digitalization dynamics in Integrated Assessment Models, one of the main tools available to generate decarbonization pathways.
The third objective of 2D4D is to identify no-regret, robust policy portfolios. These will be designed to ensure that digitalization unfolds in an inclusive, climate-beneficial way, and that decarbonization policies capitalize on digital technologies to support the energy transition.
Max ERC Funding
1 498 375 €
Duration
Start date: 2020-10-01, End date: 2025-09-30
Project acronym 2DTHERMS
Project Design of new thermoelectric devices based on layered and field modulated nanostructures of strongly correlated electron systems
Researcher (PI) Jose Francisco Rivadulla Fernandez
Host Institution (HI) UNIVERSIDAD DE SANTIAGO DE COMPOSTELA
Call Details Starting Grant (StG), PE3, ERC-2010-StG_20091028
Summary Design of new thermoelectric devices based on layered and field modulated nanostructures of strongly correlated electron systems
Summary
Design of new thermoelectric devices based on layered and field modulated nanostructures of strongly correlated electron systems
Max ERC Funding
1 427 190 €
Duration
Start date: 2010-11-01, End date: 2015-10-31
Project acronym 3-TOP
Project Exploring the physics of 3-dimensional topological insulators
Researcher (PI) Laurens Wigbolt Molenkamp
Host Institution (HI) JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG
Call Details Advanced Grant (AdG), PE3, ERC-2010-AdG_20100224
Summary Topological insulators constitute a novel class of materials where the topological details of the bulk band structure induce a robust surface state on the edges of the material. While transport data for 2-dimensional topological insulators have recently become available, experiments on their 3-dimensional counterparts are mainly limited to photoelectron spectroscopy. At the same time, a plethora of interesting novel physical phenomena have been predicted to occur in such systems.
In this proposal, we sketch an approach to tackle the transport and magnetic properties of the surface states in these materials. This starts with high quality layer growth, using molecular beam epitaxy, of bulk layers of HgTe, Bi2Se3 and Bi2Te3, which are the prime candidates to show the novel physics expected in this field. The existence of the relevant surface states will be assessed spectroscopically, but from there on research will focus on fabricating and characterizing nanostructures designed to elucidate the transport and magnetic properties of the topological surfaces using electrical, optical and scanning probe techniques. Apart from a general characterization of the Dirac band structure of the surface states, research will focus on the predicted magnetic monopole-like response of the system to an electrical test charge. In addition, much effort will be devoted to contacting the surface state with superconducting and magnetic top layers, with the final aim of demonstrating Majorana fermion behavior. As a final benefit, growth of thin high quality thin Bi2Se3 or Bi2Te3 layers could allow for a demonstration of the (2-dimensional) quantum spin Hall effect at room temperature - offering a road map to dissipation-less transport for the semiconductor industry.
Summary
Topological insulators constitute a novel class of materials where the topological details of the bulk band structure induce a robust surface state on the edges of the material. While transport data for 2-dimensional topological insulators have recently become available, experiments on their 3-dimensional counterparts are mainly limited to photoelectron spectroscopy. At the same time, a plethora of interesting novel physical phenomena have been predicted to occur in such systems.
In this proposal, we sketch an approach to tackle the transport and magnetic properties of the surface states in these materials. This starts with high quality layer growth, using molecular beam epitaxy, of bulk layers of HgTe, Bi2Se3 and Bi2Te3, which are the prime candidates to show the novel physics expected in this field. The existence of the relevant surface states will be assessed spectroscopically, but from there on research will focus on fabricating and characterizing nanostructures designed to elucidate the transport and magnetic properties of the topological surfaces using electrical, optical and scanning probe techniques. Apart from a general characterization of the Dirac band structure of the surface states, research will focus on the predicted magnetic monopole-like response of the system to an electrical test charge. In addition, much effort will be devoted to contacting the surface state with superconducting and magnetic top layers, with the final aim of demonstrating Majorana fermion behavior. As a final benefit, growth of thin high quality thin Bi2Se3 or Bi2Te3 layers could allow for a demonstration of the (2-dimensional) quantum spin Hall effect at room temperature - offering a road map to dissipation-less transport for the semiconductor industry.
Max ERC Funding
2 419 590 €
Duration
Start date: 2011-04-01, End date: 2016-03-31
Project acronym 3CBIOTECH
Project Cold Carbon Catabolism of Microbial Communities underprinning a Sustainable Bioenergy and Biorefinery Economy
Researcher (PI) Gavin James Collins
Host Institution (HI) NATIONAL UNIVERSITY OF IRELAND GALWAY
Call Details Starting Grant (StG), LS9, ERC-2010-StG_20091118
Summary The applicant will collaborate with Irish, European and U.S.-based colleagues to develop a sustainable biorefinery and bioenergy industry in Ireland and Europe. The focus of this ERC Starting Grant will be the application of classical microbiological, physiological and real-time polymerase chain reaction (PCR)-based assays, to qualitatively and quantitatively characterize microbial communities underpinning novel and innovative, low-temperature, anaerobic waste (and other biomass) conversion technologies, including municipal wastewater treatment and, demonstration- and full-scale biorefinery applications.
Anaerobic digestion (AD) is a naturally-occurring process, which is widely applied for the conversion of waste to methane-containing biogas. Low-temperature (<20 degrees C) AD has been applied by the applicant as a cost-effective alternative to mesophilic (c. 35C) AD for the treatment of several waste categories. However, the microbiology of low-temperature AD is poorly understood. The applicant will work with microbial consortia isolated from anaerobic bioreactors, which have been operated for long-term experiments (>3.5 years), and include organic acid-oxidizing, hydrogen-producing syntrophic microbes and hydrogen-consuming methanogens. A major focus of the project will be the ecophysiology of psychrotolerant and psychrophilic methanogens already identified and cultivated by the applicant. The project will also investigate the role(s) of poorly-understood Crenarchaeota populations and homoacetogenic bacteria, in complex consortia. The host organization is a leading player in the microbiology of waste-to-energy applications. The applicant will train a team of scientists in all aspects of the microbiology and bioengineering of biomass conversion systems.
Summary
The applicant will collaborate with Irish, European and U.S.-based colleagues to develop a sustainable biorefinery and bioenergy industry in Ireland and Europe. The focus of this ERC Starting Grant will be the application of classical microbiological, physiological and real-time polymerase chain reaction (PCR)-based assays, to qualitatively and quantitatively characterize microbial communities underpinning novel and innovative, low-temperature, anaerobic waste (and other biomass) conversion technologies, including municipal wastewater treatment and, demonstration- and full-scale biorefinery applications.
Anaerobic digestion (AD) is a naturally-occurring process, which is widely applied for the conversion of waste to methane-containing biogas. Low-temperature (<20 degrees C) AD has been applied by the applicant as a cost-effective alternative to mesophilic (c. 35C) AD for the treatment of several waste categories. However, the microbiology of low-temperature AD is poorly understood. The applicant will work with microbial consortia isolated from anaerobic bioreactors, which have been operated for long-term experiments (>3.5 years), and include organic acid-oxidizing, hydrogen-producing syntrophic microbes and hydrogen-consuming methanogens. A major focus of the project will be the ecophysiology of psychrotolerant and psychrophilic methanogens already identified and cultivated by the applicant. The project will also investigate the role(s) of poorly-understood Crenarchaeota populations and homoacetogenic bacteria, in complex consortia. The host organization is a leading player in the microbiology of waste-to-energy applications. The applicant will train a team of scientists in all aspects of the microbiology and bioengineering of biomass conversion systems.
Max ERC Funding
1 499 797 €
Duration
Start date: 2011-05-01, End date: 2016-04-30
Project acronym 3D MAGiC
Project Three-dimensional magnetization textures: Discovery and control on the nanoscale
Researcher (PI) Rafal Dunin-Borkowski
Host Institution (HI) FORSCHUNGSZENTRUM JULICH GMBH
Call Details Synergy Grants (SyG), SyG3D, ERC-2019-SyG
Summary Over the past 150 years, many of the greatest questions in physics, spanning astronomical dimensions to quarks, have addressed how particles can emerge in continuous fields. In this highly exploratory project, we will open a window into the behavior and control of some of the least explored and most puzzling objects in nanomagnetism: three-dimensional (3D) magnetic solitons (MSs). These are spatially localized stable magnetization textures that have particle-like properties and are expected to move and interact in 3D in magnetic crystals and heterostructures in a similar manner to ordinary particles. Until now, their theoretical study has been restricted to simple models, while the experimental study of individual 3D MSs is nearly unexplored as a result of their deep-sub-micron size and a current lack of suitable characterization techniques. We bring together four complementary research groups with expertise in theoretical descriptions of magnetism, device physics and magnetic characterization with high spatial and temporal resolution. Methodological breakthroughs by the partners will enable new fundamental theoretical and experimental insights into the nucleation, stability, dynamics and transport of 3D MSs, which are predicted to be influenced strongly by their nontrivial topology. Particular attention will be paid to the manner in which 3D MSs can be controlled and manipulated dynamically. This project will open the field of 3D magnetization textures at the nanoscale to fundamental science,with a view to enabling disruptive applications. 3D MSs are foreseen to play the role of information carriers that can move freely in any spatial direction and to offer a key advance over conventional 2D magnetization textures. Results from the project will provide guidelines for their use in applications that include magnetic storage technology and neuromorphic information processing systems and enable the realization of pervasive new 3D device concepts.
Summary
Over the past 150 years, many of the greatest questions in physics, spanning astronomical dimensions to quarks, have addressed how particles can emerge in continuous fields. In this highly exploratory project, we will open a window into the behavior and control of some of the least explored and most puzzling objects in nanomagnetism: three-dimensional (3D) magnetic solitons (MSs). These are spatially localized stable magnetization textures that have particle-like properties and are expected to move and interact in 3D in magnetic crystals and heterostructures in a similar manner to ordinary particles. Until now, their theoretical study has been restricted to simple models, while the experimental study of individual 3D MSs is nearly unexplored as a result of their deep-sub-micron size and a current lack of suitable characterization techniques. We bring together four complementary research groups with expertise in theoretical descriptions of magnetism, device physics and magnetic characterization with high spatial and temporal resolution. Methodological breakthroughs by the partners will enable new fundamental theoretical and experimental insights into the nucleation, stability, dynamics and transport of 3D MSs, which are predicted to be influenced strongly by their nontrivial topology. Particular attention will be paid to the manner in which 3D MSs can be controlled and manipulated dynamically. This project will open the field of 3D magnetization textures at the nanoscale to fundamental science,with a view to enabling disruptive applications. 3D MSs are foreseen to play the role of information carriers that can move freely in any spatial direction and to offer a key advance over conventional 2D magnetization textures. Results from the project will provide guidelines for their use in applications that include magnetic storage technology and neuromorphic information processing systems and enable the realization of pervasive new 3D device concepts.
Max ERC Funding
11 880 356 €
Duration
Start date: 2020-07-01, End date: 2026-06-30
Project acronym 3D-In-Macro
Project Inequality in 3D – measurement and implications for macroeconomic theory
Researcher (PI) Andreas Fagereng
Host Institution (HI) STIFTELSEN HANDELSHOYSKOLEN BI
Call Details Starting Grant (StG), SH1, ERC-2019-STG
Summary This project will contribute toward a better understanding of inequality and its macroeconomic implications. We will study inequality and its dynamics along three dimensions: Consumption, Income and Wealth, “3D Inequality.” With novel microdata we can measure the entirety of the economy down to the single household along the 3 dimensions.
In macroeconomics, much theoretical progress has been made in understanding when distributions matter for aggregates. Newer heterogeneous agent models deliver strikingly different implications for monetary and fiscal policies than what the traditional representative agent models do, and also allow us to study the distributional implications of different policies across households. In principle, this class of models can incorporate the potentially rich interactions between inequality and the macroeconomy: on the one hand, inequality shapes macroeconomic aggregates; on the other hand, macroeconomic shocks and policies affect inequality. However, absent precise micro-level facts it is difficult to establish which of the potential mechanisms highlighted by these models are the most important in reality.
Our empirical efforts will be disciplined by these recent developments in modelling macroeconomic phenomena with microeconomic heterogeneity. Our overarching motivation is to quantify the type of micro heterogeneity that matters for macroeconomic theory and thereby inform the development of current and future macroeconomic models. The novel insights we aim to provide could lead to substantial improvements in both fiscal and monetary policy tools. Furthermore, a better understanding of the forces behind growing inequality will inform the current debate on this issue and provide important lessons to policy makers who see economic inequality as a problem in itself.
Summary
This project will contribute toward a better understanding of inequality and its macroeconomic implications. We will study inequality and its dynamics along three dimensions: Consumption, Income and Wealth, “3D Inequality.” With novel microdata we can measure the entirety of the economy down to the single household along the 3 dimensions.
In macroeconomics, much theoretical progress has been made in understanding when distributions matter for aggregates. Newer heterogeneous agent models deliver strikingly different implications for monetary and fiscal policies than what the traditional representative agent models do, and also allow us to study the distributional implications of different policies across households. In principle, this class of models can incorporate the potentially rich interactions between inequality and the macroeconomy: on the one hand, inequality shapes macroeconomic aggregates; on the other hand, macroeconomic shocks and policies affect inequality. However, absent precise micro-level facts it is difficult to establish which of the potential mechanisms highlighted by these models are the most important in reality.
Our empirical efforts will be disciplined by these recent developments in modelling macroeconomic phenomena with microeconomic heterogeneity. Our overarching motivation is to quantify the type of micro heterogeneity that matters for macroeconomic theory and thereby inform the development of current and future macroeconomic models. The novel insights we aim to provide could lead to substantial improvements in both fiscal and monetary policy tools. Furthermore, a better understanding of the forces behind growing inequality will inform the current debate on this issue and provide important lessons to policy makers who see economic inequality as a problem in itself.
Max ERC Funding
1 376 875 €
Duration
Start date: 2020-05-01, End date: 2025-04-30
Project acronym 3D-loop
Project Mechanism of homology search and the logic of homologous chromosome pairing in meiosis
Researcher (PI) Aurele PIAZZA
Host Institution (HI) CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Call Details Starting Grant (StG), LS2, ERC-2019-STG
Summary Homologous recombination (HR) is a conserved DNA double-strand breaks (DSB) repair pathway that uniquely uses an intact DNA molecule as a template. Genome-wide homology search is carried out by a nucleoprotein filament (NPF) assembled on the ssDNA flanking the DSB, and whose product is a “D-loop” joint molecule. Beyond accurate DSB repair, this capacity of HR to spatially associates homologous molecules is also harnessed for homolog pairing in meiosis. The goal of “3D-loop” is to tackle two long lasting conundrums: the fundamental homology search mechanism that achieves accurate and efficient identification of a single homologous donor in the vastness of the genome and nucleus, and how this mechanism is adapted for the purpose of homologs attachment in meiosis.
I overcame the main hurdle to study these core steps of HR by developing a suite of proximity ligation-based methodologies and experimental systems to physically detect joint molecules in yeast cells. It revealed elaborate regulation controlling D-loop dynamics and a novel class of joint molecules. This proposal builds upon these methodologies and findings to first address basic properties of the homology sampling process by the NPF and the role of D-loop dynamics, with the long-term goal to establish a quantitative framework of homology search in mitotic cells (WP1). Second, the meiosis-specific regulation of homology search leading to homolog pairing likely integrates chromosomal-scale information. Genome re-synthesis and engineering approaches will be deployed to (i) achieve a quantitative and dynamic cartography of the cytological and molecular events of meiosis over a large chromosomal region, (ii) probe cis-acting regulations at the chromosomal scale, and (iii) revisit the molecular paradigm for crossover formation (WP2). We expect this project to shed light on the fundamental process of homology search and its involvement in the chromosome pairing phenomenon lying at the basis of sexual reproduction.
Summary
Homologous recombination (HR) is a conserved DNA double-strand breaks (DSB) repair pathway that uniquely uses an intact DNA molecule as a template. Genome-wide homology search is carried out by a nucleoprotein filament (NPF) assembled on the ssDNA flanking the DSB, and whose product is a “D-loop” joint molecule. Beyond accurate DSB repair, this capacity of HR to spatially associates homologous molecules is also harnessed for homolog pairing in meiosis. The goal of “3D-loop” is to tackle two long lasting conundrums: the fundamental homology search mechanism that achieves accurate and efficient identification of a single homologous donor in the vastness of the genome and nucleus, and how this mechanism is adapted for the purpose of homologs attachment in meiosis.
I overcame the main hurdle to study these core steps of HR by developing a suite of proximity ligation-based methodologies and experimental systems to physically detect joint molecules in yeast cells. It revealed elaborate regulation controlling D-loop dynamics and a novel class of joint molecules. This proposal builds upon these methodologies and findings to first address basic properties of the homology sampling process by the NPF and the role of D-loop dynamics, with the long-term goal to establish a quantitative framework of homology search in mitotic cells (WP1). Second, the meiosis-specific regulation of homology search leading to homolog pairing likely integrates chromosomal-scale information. Genome re-synthesis and engineering approaches will be deployed to (i) achieve a quantitative and dynamic cartography of the cytological and molecular events of meiosis over a large chromosomal region, (ii) probe cis-acting regulations at the chromosomal scale, and (iii) revisit the molecular paradigm for crossover formation (WP2). We expect this project to shed light on the fundamental process of homology search and its involvement in the chromosome pairing phenomenon lying at the basis of sexual reproduction.
Max ERC Funding
1 499 779 €
Duration
Start date: 2020-01-01, End date: 2024-12-31
Project acronym 3D-PIV
Project Valorization trajectory of a 3D particle image velocimetry instrument
Researcher (PI) Wim DE MALSCHE
Host Institution (HI) VRIJE UNIVERSITEIT BRUSSEL
Call Details Proof of Concept (PoC), ERC-2019-PoC
Summary Actual implementation of impactful applications for microfluidic devices in a commercial setting has been surprisingly limited so far. The cause can be to a great extent attributed to the main feature of microfluidic devices: their small dimensions. While miniaturized structures are essential in generating key functionalities, they are also ideal nucleation and anchor sites for solid material present in the liquid that flows through the channels, a phenomenon called fouling. This subsequently results in a reduced or loss of functionality and eventually plugging of the entire flow system. The solution to avoiding fouling is measuring the flow in microfluidic devices in 3D, by particle image velocimetry (PIV), either when designing or using them. However, achieving 3D imaging of flows is currently an extremely difficult task due to the amount of work, high costs and lengthy timelines required. Our value proposition in the ERC Proof of Concept project ‘3D-PIV’ is a table-top device able to efficiently analyse the velocimetry of particles in 3D, offering an unprecedented level of detail of the fluid motion through micron-sized channels/inlets/outlets, opening new possibilities in microfluidics design and validation with significant impact on multiple applications. One of the killer applications we envision, and our focus in this ERC Proof of Concept project, is in the pharmaceutical and chemical industries, for the manufacturing of drugs or chemical components, to enable, adjust or improve their separation. In this project we will focus on building a strong business case for our 3D-PIV technology through prototyping, optimizing software, market analysis and business development.
Summary
Actual implementation of impactful applications for microfluidic devices in a commercial setting has been surprisingly limited so far. The cause can be to a great extent attributed to the main feature of microfluidic devices: their small dimensions. While miniaturized structures are essential in generating key functionalities, they are also ideal nucleation and anchor sites for solid material present in the liquid that flows through the channels, a phenomenon called fouling. This subsequently results in a reduced or loss of functionality and eventually plugging of the entire flow system. The solution to avoiding fouling is measuring the flow in microfluidic devices in 3D, by particle image velocimetry (PIV), either when designing or using them. However, achieving 3D imaging of flows is currently an extremely difficult task due to the amount of work, high costs and lengthy timelines required. Our value proposition in the ERC Proof of Concept project ‘3D-PIV’ is a table-top device able to efficiently analyse the velocimetry of particles in 3D, offering an unprecedented level of detail of the fluid motion through micron-sized channels/inlets/outlets, opening new possibilities in microfluidics design and validation with significant impact on multiple applications. One of the killer applications we envision, and our focus in this ERC Proof of Concept project, is in the pharmaceutical and chemical industries, for the manufacturing of drugs or chemical components, to enable, adjust or improve their separation. In this project we will focus on building a strong business case for our 3D-PIV technology through prototyping, optimizing software, market analysis and business development.
Max ERC Funding
150 000 €
Duration
Start date: 2020-01-01, End date: 2021-06-30