Project acronym EIRENE
Project Post-war trasistions in gendered perspective: the case of the North-Eastern Adricatic Region
Researcher (PI) Marta VERGINELLA
Host Institution (HI) UNIVERZA V LJUBLJANI
Call Details Advanced Grant (AdG), SH6, ERC-2016-ADG
Summary The EIRENE project’s purpose is to think afresh 20th-century post-war transitions by taking into account a gendered perspective. Namely, the historiographic consideration of gender thoroughly alters the understanding of social dynamics in multi-ethnic areas during the post-war transitions. They will be observed in the North-Eastern Adriatic region, an overlooked European space, marked by border redefinitions, changes of political systems, and high interethnic conflict intensity, but also by genuine cooperation among ethnic groups. The region has all the qualities of a “laboratory environment” for the study of gender positions and interrelations after World Wars I and II and after the Yugoslav wars in the 1990s. The project will differ substantially from previous attempts to analyse post-war transitions in these aspects: a) longitudinal approach, comparing three post-war periods in order to detect their specifics and (dis)continuities; b) transnational approach, by overcoming nation-centric frameworks of analysis; c) by combining conceptual political and social sciences with historiography; and finally, d) by examining post-war transitions through the prism of gender. Focusing on four research-fields (politics, political violence, work, family), the project will validate innovative analytical concepts of the “inclusion-exclusion paradox” of women in post-war transitions, and women as “cross-boundary mediators”. Within the category of gender, focal attention will be given to women as they are often invisible in historical accounts and remain neglected in historicizing. By aggregating empirical sources, the project will approach the proposed subject matter by investigating the processes of identification across the lines of ethnic origin, class, generations, marital status, profession/occupation, language of use, migratory processes, etc. The project’s added value is its novel conceptual applicability to other comparable geopolitical areas.
Summary
The EIRENE project’s purpose is to think afresh 20th-century post-war transitions by taking into account a gendered perspective. Namely, the historiographic consideration of gender thoroughly alters the understanding of social dynamics in multi-ethnic areas during the post-war transitions. They will be observed in the North-Eastern Adriatic region, an overlooked European space, marked by border redefinitions, changes of political systems, and high interethnic conflict intensity, but also by genuine cooperation among ethnic groups. The region has all the qualities of a “laboratory environment” for the study of gender positions and interrelations after World Wars I and II and after the Yugoslav wars in the 1990s. The project will differ substantially from previous attempts to analyse post-war transitions in these aspects: a) longitudinal approach, comparing three post-war periods in order to detect their specifics and (dis)continuities; b) transnational approach, by overcoming nation-centric frameworks of analysis; c) by combining conceptual political and social sciences with historiography; and finally, d) by examining post-war transitions through the prism of gender. Focusing on four research-fields (politics, political violence, work, family), the project will validate innovative analytical concepts of the “inclusion-exclusion paradox” of women in post-war transitions, and women as “cross-boundary mediators”. Within the category of gender, focal attention will be given to women as they are often invisible in historical accounts and remain neglected in historicizing. By aggregating empirical sources, the project will approach the proposed subject matter by investigating the processes of identification across the lines of ethnic origin, class, generations, marital status, profession/occupation, language of use, migratory processes, etc. The project’s added value is its novel conceptual applicability to other comparable geopolitical areas.
Max ERC Funding
2 266 067 €
Duration
Start date: 2017-12-01, End date: 2022-11-30
Project acronym MaCChines
Project Molecular machines based on coiled-coil protein origami
Researcher (PI) Roman JERALA
Host Institution (HI) KEMIJSKI INSTITUT
Call Details Advanced Grant (AdG), LS9, ERC-2017-ADG
Summary Proteins are the most versatile and complex smart nanomaterials, forming molecular machines and performing numerous functions from structure building, recognition, catalysis to locomotion. Nature however explored only a tiny fraction of possible protein sequences and structures. Design of proteins with new, in nature unseen shapes and features, offers high rewards for medicine, technology and science. In 2013 my group pioneered the design of a new type of modular coiled-coil protein origami (CCPO) folds. This type of de novo designed proteins are defined by the sequence of coiled-coil (CC) dimer-forming modules that are concatenated by flexible linkers into a single polypeptide chain that self-assembles into a polyhedral cage based on pairwise CC interactions. This is in contrast to naturally evolved proteins where their fold is defined by a compact hydrophobic core. We recently demonstrated the robustness of this strategy by the largest de novo designed single chain protein, construction of tetrahedral, pyramid, trigonal prism and bipyramid cages that self-assemble in vivo.
This proposal builds on unique advantages of CCPOs and represents a new frontier of this branch of protein design science. I propose to introduce functional domains into selected positions of CCPO cages, implement new types of building modules that will enable regulated CCPO assembly and disassembly, test new strategies of caging and release of cargo molecules for targeted delivery, design knotted and crosslinked protein cages and introduce toehold displacement for the regulated structural rearrangement of CCPOs required for designed molecular machines, which will be demonstrated on protein nanotweezers. Technology for the positional combinatorial library-based single pot assembly of CCPO genes will provide high throughput of CCPO variants. Project will result in new methodology, understanding of potentials of CCPOs for designed molecular machines and in demonstration of different applications.
Summary
Proteins are the most versatile and complex smart nanomaterials, forming molecular machines and performing numerous functions from structure building, recognition, catalysis to locomotion. Nature however explored only a tiny fraction of possible protein sequences and structures. Design of proteins with new, in nature unseen shapes and features, offers high rewards for medicine, technology and science. In 2013 my group pioneered the design of a new type of modular coiled-coil protein origami (CCPO) folds. This type of de novo designed proteins are defined by the sequence of coiled-coil (CC) dimer-forming modules that are concatenated by flexible linkers into a single polypeptide chain that self-assembles into a polyhedral cage based on pairwise CC interactions. This is in contrast to naturally evolved proteins where their fold is defined by a compact hydrophobic core. We recently demonstrated the robustness of this strategy by the largest de novo designed single chain protein, construction of tetrahedral, pyramid, trigonal prism and bipyramid cages that self-assemble in vivo.
This proposal builds on unique advantages of CCPOs and represents a new frontier of this branch of protein design science. I propose to introduce functional domains into selected positions of CCPO cages, implement new types of building modules that will enable regulated CCPO assembly and disassembly, test new strategies of caging and release of cargo molecules for targeted delivery, design knotted and crosslinked protein cages and introduce toehold displacement for the regulated structural rearrangement of CCPOs required for designed molecular machines, which will be demonstrated on protein nanotweezers. Technology for the positional combinatorial library-based single pot assembly of CCPO genes will provide high throughput of CCPO variants. Project will result in new methodology, understanding of potentials of CCPOs for designed molecular machines and in demonstration of different applications.
Max ERC Funding
2 497 125 €
Duration
Start date: 2018-09-01, End date: 2023-08-31
Project acronym OMNES
Project Open Many-body Non-Equilibrium Systems
Researcher (PI) Tomaz PROSEN
Host Institution (HI) UNIVERZA V LJUBLJANI
Call Details Advanced Grant (AdG), PE3, ERC-2015-AdG
Summary We shall study non-equilibrium many-body quantum systems, considering local interactions in one or two spatial dimensions in situations where the generator of time evolution in the bulk of the system is unitary whereas the incoherent processes are limited to the system's boundaries. We foresee a mathematical theory of dynamical quantum phases of matter with applications in the theory of quantum transport and nanoscale devices that manipulate heat, information, charge or magnetization.
Our steady-state setup represents a fundamental paradigm of mathematical statistical physics which has been pioneered by the PI, who gave the first explicit solution for boundary driven/dissipative strongly interacting many-body problem (XXZ spin 1/2 chain) which answered a long debated question on strict positivity of the spin Drude weight at high temperature.
The main focus of OMNES will be centered on exploring the following three interconnected pathways: Most importantly, we shall develop a general framework for exact solutions of non-equilibrium integrable quantum many-body models, in particular the steady states and relaxation modes, and develop quantum integrability methods for non-equilibrium many-body density operators. Fundamentally new concepts which are expected to emerge from these studies, relevant beyond the context of boundary-driven/dissipative systems, are novel quasilocal conservation laws of the bulk Hamiltonian dynamics. Second, we shall investigate relevance of exact solutions in physics of generic systems which are small perturbations of integrable models and explore the problem of stability of local and quasilocal conserved quantities under generic integrability-breaking perturbations. Third, we shall formulate and study the problem of quantum chaos in clean lattice systems, in particular to establish a link between random matrix theory of level statistics and kinematic and dynamical features of lattice models with sufficiently strong integrability breaking.
Summary
We shall study non-equilibrium many-body quantum systems, considering local interactions in one or two spatial dimensions in situations where the generator of time evolution in the bulk of the system is unitary whereas the incoherent processes are limited to the system's boundaries. We foresee a mathematical theory of dynamical quantum phases of matter with applications in the theory of quantum transport and nanoscale devices that manipulate heat, information, charge or magnetization.
Our steady-state setup represents a fundamental paradigm of mathematical statistical physics which has been pioneered by the PI, who gave the first explicit solution for boundary driven/dissipative strongly interacting many-body problem (XXZ spin 1/2 chain) which answered a long debated question on strict positivity of the spin Drude weight at high temperature.
The main focus of OMNES will be centered on exploring the following three interconnected pathways: Most importantly, we shall develop a general framework for exact solutions of non-equilibrium integrable quantum many-body models, in particular the steady states and relaxation modes, and develop quantum integrability methods for non-equilibrium many-body density operators. Fundamentally new concepts which are expected to emerge from these studies, relevant beyond the context of boundary-driven/dissipative systems, are novel quasilocal conservation laws of the bulk Hamiltonian dynamics. Second, we shall investigate relevance of exact solutions in physics of generic systems which are small perturbations of integrable models and explore the problem of stability of local and quasilocal conserved quantities under generic integrability-breaking perturbations. Third, we shall formulate and study the problem of quantum chaos in clean lattice systems, in particular to establish a link between random matrix theory of level statistics and kinematic and dynamical features of lattice models with sufficiently strong integrability breaking.
Max ERC Funding
2 041 000 €
Duration
Start date: 2016-10-01, End date: 2021-09-30
Project acronym RNPdynamics
Project Multivalent interactions driving RNP dynamics in development and disease
Researcher (PI) Jernej ULE
Host Institution (HI) KEMIJSKI INSTITUT
Call Details Advanced Grant (AdG), LS2, ERC-2018-ADG
Summary Ribonucleoprotein complexes (RNPs) play many key regulatory roles in development. Moreover, mutations causing cancer or neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS), often occur in RNA-binding proteins (RBPs). These mutations are concentrated in the intrinsically disordered regions (IDRs), which play a central role in the control of RNP assembly and disassembly. RNP dynamics is often driven by multivalent interactions that are mediated by multiple elements within IDRs of RBPs, which can condense the RNP such that it separates from the surrounding liquid through the phenomenon of liquid-liquid phase separation. Transcriptomic insights into the physiological functions of such multivalent RNP assembly are needed to understand their regulation, or deregulation through disease-causing mutations. Here, we will build a framework of experimental and computational methods to study the mechanisms by which the dynamic multivalent interactions drive RNP remodelling, and how such RNP dynamics contributes to cellular transitions in development and disease. The first objective will be to identify the functions of specific RBPs in cell-state transitions during neuronal differentiation, and the mechanisms of IDR-mediated multivalent interactions in these functions. The next objective will be to establish new tools to manipulate RNP assembly through multivalent RNA binding sites and IDRs. Finally, the new insights and tools will be integrated with the goal to fine-tune the RNP assembly of ALS-mutant RBPs, and thereby ameliorate their toxicity.
Summary
Ribonucleoprotein complexes (RNPs) play many key regulatory roles in development. Moreover, mutations causing cancer or neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS), often occur in RNA-binding proteins (RBPs). These mutations are concentrated in the intrinsically disordered regions (IDRs), which play a central role in the control of RNP assembly and disassembly. RNP dynamics is often driven by multivalent interactions that are mediated by multiple elements within IDRs of RBPs, which can condense the RNP such that it separates from the surrounding liquid through the phenomenon of liquid-liquid phase separation. Transcriptomic insights into the physiological functions of such multivalent RNP assembly are needed to understand their regulation, or deregulation through disease-causing mutations. Here, we will build a framework of experimental and computational methods to study the mechanisms by which the dynamic multivalent interactions drive RNP remodelling, and how such RNP dynamics contributes to cellular transitions in development and disease. The first objective will be to identify the functions of specific RBPs in cell-state transitions during neuronal differentiation, and the mechanisms of IDR-mediated multivalent interactions in these functions. The next objective will be to establish new tools to manipulate RNP assembly through multivalent RNA binding sites and IDRs. Finally, the new insights and tools will be integrated with the goal to fine-tune the RNP assembly of ALS-mutant RBPs, and thereby ameliorate their toxicity.
Max ERC Funding
2 396 261 €
Duration
Start date: 2020-01-01, End date: 2024-12-31
Project acronym TRAJECTORY
Project Coherent trajectories through symmetry breaking transitions
Researcher (PI) Dragan Mihailovic
Host Institution (HI) INSTITUT JOZEF STEFAN
Call Details Advanced Grant (AdG), PE3, ERC-2012-ADG_20120216
Summary We propose to investigate the coherent trajectories of many-body systems undergoing symmetry-breaking transitions (SBTs) in real time, where trajectories are meant here in a mathematical sense used to describe the dynamics of nonlinear systems. The key idea which makes this project possible is the development of a specific femtosecond laser spectroscopy technique which allows us to distinguish the order parameter dynamics in complex matter systems from hot-electron energy relaxation, quasiparticle recombination processes, damping and dephasing of coherent phonon oscillations. This allows real-time high resolution investigations of the critical system trajectories through SBTs, beyond the capabilities of current state of the art time-resolved techniques. We will investigate coherent collective field oscillations and the fundamentals of topological defect creation by the Kibble-Zurek mechanism including a study of their annihilation in the aftermath of SBTs. We will aim to control the coherent trajectories at bifurcation points by laser pulses and external fields. We will address fundamental questions on the effect of symmetry and fundamental interactions of underlying microscopic vacua on global behaviour. Systems included in our study belong to a number of different universality classes and include the study of nontrivial transitions to newly discovered hidden states of matter. In the general framework of reductionism, we expect our findings to have fundamental bearing on our understanding of SBTs revealing predictive tell-tale signatures of critical events of relevance in areas beyond many-body condensed matter physics, in elementary particle physics, primordial cosmological events and tipping points in nonlinear systems. Transition trajectories to and from hidden states are of particular interest for practical applications in new femtosecond state change memory devices.
Summary
We propose to investigate the coherent trajectories of many-body systems undergoing symmetry-breaking transitions (SBTs) in real time, where trajectories are meant here in a mathematical sense used to describe the dynamics of nonlinear systems. The key idea which makes this project possible is the development of a specific femtosecond laser spectroscopy technique which allows us to distinguish the order parameter dynamics in complex matter systems from hot-electron energy relaxation, quasiparticle recombination processes, damping and dephasing of coherent phonon oscillations. This allows real-time high resolution investigations of the critical system trajectories through SBTs, beyond the capabilities of current state of the art time-resolved techniques. We will investigate coherent collective field oscillations and the fundamentals of topological defect creation by the Kibble-Zurek mechanism including a study of their annihilation in the aftermath of SBTs. We will aim to control the coherent trajectories at bifurcation points by laser pulses and external fields. We will address fundamental questions on the effect of symmetry and fundamental interactions of underlying microscopic vacua on global behaviour. Systems included in our study belong to a number of different universality classes and include the study of nontrivial transitions to newly discovered hidden states of matter. In the general framework of reductionism, we expect our findings to have fundamental bearing on our understanding of SBTs revealing predictive tell-tale signatures of critical events of relevance in areas beyond many-body condensed matter physics, in elementary particle physics, primordial cosmological events and tipping points in nonlinear systems. Transition trajectories to and from hidden states are of particular interest for practical applications in new femtosecond state change memory devices.
Max ERC Funding
1 503 600 €
Duration
Start date: 2013-05-01, End date: 2018-04-30
