Project acronym BIOMOLECULAR_COMP
Project Biomolecular computers
Researcher (PI) Ehud Shapiro
Host Institution (HI) WEIZMANN INSTITUTE OF SCIENCE
Call Details Advanced Grant (AdG), LS9, ERC-2008-AdG
Summary Autonomous programmable computing devices made of biological molecules hold the promise of interacting with the biological environment in future biological and medical applications. Our laboratory's long-term objective is to develop a 'Doctor in a cell': molecular-sized device that can roam the body, equipped with medical knowledge. It would diagnose a disease by analyzing the data available in its biochemical environment based on the encoded medical knowledge and treat it by releasing the appropriate drug molecule in situ. This kind of device might, in the future, be delivered to all cells in a specific tissue, organ or the whole organism, and cure or kill only those cells diagnosed with a disease. Our laboratory embarked on the attempt to design and build these molecular computing devices and lay the foundation for their future biomedical applications. Several important milestones have already been accomplished towards the realization of the Doctor in a cell vision. The subject of this proposal is a construction of autonomous biomolecular computers that could be delivered into a living cell, interact with endogenous biomolecules that are known to indicate diseases, logically analyze them, make a diagnostic decision and couple it to the production of an active biomolecule capable of influencing cell fate.
Summary
Autonomous programmable computing devices made of biological molecules hold the promise of interacting with the biological environment in future biological and medical applications. Our laboratory's long-term objective is to develop a 'Doctor in a cell': molecular-sized device that can roam the body, equipped with medical knowledge. It would diagnose a disease by analyzing the data available in its biochemical environment based on the encoded medical knowledge and treat it by releasing the appropriate drug molecule in situ. This kind of device might, in the future, be delivered to all cells in a specific tissue, organ or the whole organism, and cure or kill only those cells diagnosed with a disease. Our laboratory embarked on the attempt to design and build these molecular computing devices and lay the foundation for their future biomedical applications. Several important milestones have already been accomplished towards the realization of the Doctor in a cell vision. The subject of this proposal is a construction of autonomous biomolecular computers that could be delivered into a living cell, interact with endogenous biomolecules that are known to indicate diseases, logically analyze them, make a diagnostic decision and couple it to the production of an active biomolecule capable of influencing cell fate.
Max ERC Funding
2 125 980 €
Duration
Start date: 2009-01-01, End date: 2013-10-31
Project acronym DMMCA
Project Discrete Mathematics: methods, challenges and applications
Researcher (PI) Noga Alon
Host Institution (HI) TEL AVIV UNIVERSITY
Call Details Advanced Grant (AdG), PE1, ERC-2008-AdG
Summary Discrete Mathematics is a fundamental mathematical discipline as well as an essential component of many mathematical areas, and its study has experienced an impressive growth in recent years. Some of the main reasons for this growth are the broad applications of tools and techniques from extremal and probabilistic combinatorics in the rapid development of theoretical Computer Science, in the spectacular recent results in Additive Number Theory and in the study of basic questions in Information Theory. While in the past many of the basic combinatorial results were obtained mainly by ingenuity and detailed reasoning, the modern theory has grown out of this early stage, and often relies on deep, well developed tools, like the probabilistic method, algebraic, topological and geometric techniques. The work of the principal investigator, partly jointly with several collaborators and students, and partly in individual efforts, has played a significant role in the introduction of powerful algebraic, probabilistic, spectral and geometric techniques that influenced the development of modern combinatorics. In the present project he aims to try and further develop such tools, trying to tackle some basic open problems in Combinatorics, as well as significant questions in Additive Combinatorics, Information Theory, and theoretical Computer Science. Progress on the problems mentioned in this proposal, and the study of related ones, is expected to provide new insights on these problems and to lead to the development of novel fruitful techniques that are likely to be useful in Discrete Mathematics as well as in related areas.
Summary
Discrete Mathematics is a fundamental mathematical discipline as well as an essential component of many mathematical areas, and its study has experienced an impressive growth in recent years. Some of the main reasons for this growth are the broad applications of tools and techniques from extremal and probabilistic combinatorics in the rapid development of theoretical Computer Science, in the spectacular recent results in Additive Number Theory and in the study of basic questions in Information Theory. While in the past many of the basic combinatorial results were obtained mainly by ingenuity and detailed reasoning, the modern theory has grown out of this early stage, and often relies on deep, well developed tools, like the probabilistic method, algebraic, topological and geometric techniques. The work of the principal investigator, partly jointly with several collaborators and students, and partly in individual efforts, has played a significant role in the introduction of powerful algebraic, probabilistic, spectral and geometric techniques that influenced the development of modern combinatorics. In the present project he aims to try and further develop such tools, trying to tackle some basic open problems in Combinatorics, as well as significant questions in Additive Combinatorics, Information Theory, and theoretical Computer Science. Progress on the problems mentioned in this proposal, and the study of related ones, is expected to provide new insights on these problems and to lead to the development of novel fruitful techniques that are likely to be useful in Discrete Mathematics as well as in related areas.
Max ERC Funding
1 061 300 €
Duration
Start date: 2008-12-01, End date: 2013-11-30
Project acronym EXPANDERS
Project Expander Graphs in Pure and Applied Mathematics
Researcher (PI) Alexander Lubotzky
Host Institution (HI) THE HEBREW UNIVERSITY OF JERUSALEM
Call Details Advanced Grant (AdG), PE1, ERC-2008-AdG
Summary Expander graphs are finite graphs which play a fundamental role in many areas of computer science such as: communication networks, algorithms and more. Several areas of deep mathematics have been used in order to give explicit constructions of such graphs e.g. Kazhdan property (T) from representation theory of semisimple Lie groups, Ramanujan conjecture from the theory of automorphic forms and more. In recent years, computer science has started to pay its debt to mathematics: expander graphs are playing an increasing role in several areas of pure mathematics. The goal of the current research plan is to deepen these connections in both directions with special emphasis of the more recent and surprising application of expanders to group theory, the geometry of 3-manifolds and number theory.
Summary
Expander graphs are finite graphs which play a fundamental role in many areas of computer science such as: communication networks, algorithms and more. Several areas of deep mathematics have been used in order to give explicit constructions of such graphs e.g. Kazhdan property (T) from representation theory of semisimple Lie groups, Ramanujan conjecture from the theory of automorphic forms and more. In recent years, computer science has started to pay its debt to mathematics: expander graphs are playing an increasing role in several areas of pure mathematics. The goal of the current research plan is to deepen these connections in both directions with special emphasis of the more recent and surprising application of expanders to group theory, the geometry of 3-manifolds and number theory.
Max ERC Funding
1 082 504 €
Duration
Start date: 2008-10-01, End date: 2014-09-30
Project acronym FQHE
Project Statistics of Fractionally Charged Quasi-Particles
Researcher (PI) Mordehai (Moty) Heiblum
Host Institution (HI) WEIZMANN INSTITUTE OF SCIENCE
Call Details Advanced Grant (AdG), PE3, ERC-2008-AdG
Summary The discovery of the fractional quantum Hall effect created a revolution in solid state research by introducing a new state of matter resulting from strong electron interactions. The new state is characterized by excitations (quasi-particles) that carry fractional charge, which are expected to obey fractional statistics. While odd denominator fractional states are expected to have an abelian statistics, the newly discovered 5/2 even denominator fractional state is expected to have a non-abelian statistics. Moreover, a large number of emerging proposals predict that the latter state can be employed for topological quantum computing ( Station Q was founded by Microsoft Corp. in order to pursue this goal). This proposal aims at studying the abelian and non-abelian fractional charges, and in particular to observe their peculiar statistics. While charges are preferably determined by measuring quantum shot noise, their statistics must be determined via interference experiments, where one particle goes around another. The experiments are very demanding since the even denominator fractions turn to be very fragile and thus can be observed only in the purest possible two dimensional electron gas and at the lowest temperatures. While until very recently such high quality samples were available only by a single grower (in the USA), we have the capability now to grow extremely pure samples with profound even denominator states. As will be detailed in the proposal, we have all the necessary tools to study charge and statistics of these fascinating excitations, due to our experience in crystal growth, shot noise and interferometry measurements.
Summary
The discovery of the fractional quantum Hall effect created a revolution in solid state research by introducing a new state of matter resulting from strong electron interactions. The new state is characterized by excitations (quasi-particles) that carry fractional charge, which are expected to obey fractional statistics. While odd denominator fractional states are expected to have an abelian statistics, the newly discovered 5/2 even denominator fractional state is expected to have a non-abelian statistics. Moreover, a large number of emerging proposals predict that the latter state can be employed for topological quantum computing ( Station Q was founded by Microsoft Corp. in order to pursue this goal). This proposal aims at studying the abelian and non-abelian fractional charges, and in particular to observe their peculiar statistics. While charges are preferably determined by measuring quantum shot noise, their statistics must be determined via interference experiments, where one particle goes around another. The experiments are very demanding since the even denominator fractions turn to be very fragile and thus can be observed only in the purest possible two dimensional electron gas and at the lowest temperatures. While until very recently such high quality samples were available only by a single grower (in the USA), we have the capability now to grow extremely pure samples with profound even denominator states. As will be detailed in the proposal, we have all the necessary tools to study charge and statistics of these fascinating excitations, due to our experience in crystal growth, shot noise and interferometry measurements.
Max ERC Funding
2 000 000 €
Duration
Start date: 2009-01-01, End date: 2013-12-31
Project acronym IMAGO
Project Imaging regulatory pathways of angiogenesis
Researcher (PI) Michal Neeman
Host Institution (HI) WEIZMANN INSTITUTE OF SCIENCE
Call Details Advanced Grant (AdG), LS7, ERC-2008-AdG
Summary Homeostasis of multicellular tissues relies on accurate match of vascular supply and drain to the needs of the tissue. Multiple pathways are involved in detection, signalling and execution of the required steps involved in organization of blood and lymphatic vessels during embryonic development. Similar mechanisms are utilized for overcoming changes in tissue requirements also in adult tissues and in pathological processes. The goal of this work is to reveal the dynamic forces that shape the blood vessels during angiogenesis. In particular, we would like to explore the impact of interstitial convective flow in dynamic imprinting of growth factor signalling, thereby regulating vascular patterning. Angiogenesis is explored here as an example for a possible general role for interstitial convection of growth factors in determination of the fine spatial patterning of tissue morphogenesis in vertebrates. To achieve this goal, we will develop multi-modality tools for imaging the regulation of vascular patterning. In vivo imaging will then be utilized for mapping vascular patterning in pathological and physiological angiogenesis including tumours, wound repair, the preovulatory ovarian follicle and foetal implantation sites. Whole body optical, CT, ultrasound and MRI will be applied for non-invasive imaging of deep organs. Microscopic morphometric and molecular information will be derived from the macroscopic imaging data, using selective molecular imaging approaches and functional imaging tools with specific pharmacological models that will be developed to account for interstitial convective flow. Intravital two photon microscopy and fluorescence endoscopy will be used for high resolution evaluation of vascular patterning. The evaluation of novel mechanisms for spatial regulation of intercellular growth factor signalling, will allow us to define new potential targets for intervention, and to develop new tools for preclinical and clinical imaging of angiogenesis.
Summary
Homeostasis of multicellular tissues relies on accurate match of vascular supply and drain to the needs of the tissue. Multiple pathways are involved in detection, signalling and execution of the required steps involved in organization of blood and lymphatic vessels during embryonic development. Similar mechanisms are utilized for overcoming changes in tissue requirements also in adult tissues and in pathological processes. The goal of this work is to reveal the dynamic forces that shape the blood vessels during angiogenesis. In particular, we would like to explore the impact of interstitial convective flow in dynamic imprinting of growth factor signalling, thereby regulating vascular patterning. Angiogenesis is explored here as an example for a possible general role for interstitial convection of growth factors in determination of the fine spatial patterning of tissue morphogenesis in vertebrates. To achieve this goal, we will develop multi-modality tools for imaging the regulation of vascular patterning. In vivo imaging will then be utilized for mapping vascular patterning in pathological and physiological angiogenesis including tumours, wound repair, the preovulatory ovarian follicle and foetal implantation sites. Whole body optical, CT, ultrasound and MRI will be applied for non-invasive imaging of deep organs. Microscopic morphometric and molecular information will be derived from the macroscopic imaging data, using selective molecular imaging approaches and functional imaging tools with specific pharmacological models that will be developed to account for interstitial convective flow. Intravital two photon microscopy and fluorescence endoscopy will be used for high resolution evaluation of vascular patterning. The evaluation of novel mechanisms for spatial regulation of intercellular growth factor signalling, will allow us to define new potential targets for intervention, and to develop new tools for preclinical and clinical imaging of angiogenesis.
Max ERC Funding
2 278 344 €
Duration
Start date: 2009-01-01, End date: 2013-12-31
Project acronym IMMUNE/MEMORY AGING
Project Can immune system rejuvenation restore age-related memory loss?
Researcher (PI) Michal Eisenbach-Schwartz
Host Institution (HI) WEIZMANN INSTITUTE OF SCIENCE
Call Details Advanced Grant (AdG), LS5, ERC-2008-AdG
Summary With increased life expectancy, there has been a critical growth in the portion of the population that suffers from age-related cognitive decline and dementia. Attempts are therefore being made to find ways to slow brain-aging processes; successful therapies would have a significant impact on the quality of life of individuals, and decrease healthcare expenditures. Aging of the immune system has never been suggested as a factor in memory loss. My group formulated the concept of protective autoimmunity , suggesting a linkage between immunity and self-maintenance in the context of the brain in health and disease. Recently, we showed that T lymphocytes recognizing brain-self antigens have a pivotal role in maintaining hippocampal plasticity, as manifested by reduced neurogenesis and impaired cognitive abilities in T-cell deficient mice. Taken together, our novel observations that T cell immunity contributes to hippocampal plasticity, and the fact that T cell immunity decreases with progressive aging create the basis for the present proposal. We will focus on the following questions: (a) Which aspects of cognition are supported by the immune system- learning, memory or both; (b) whether aging of the immune system is sufficient to induce aging of the brain; (c) whether activation of the immune system is sufficient to reverse age-related cognitive decline; (d) the mechanism underlying the effect of peripheral immunity on brain cognition; and (e) potential therapeutic implications of our findings. Our preliminary results demonstrate that the immune system contributes to spatial memory, and that imposing an immune deficiency is sufficient to cause a reversible memory deficit. These findings give strong reason for optimism that memory loss in the elderly is preventable and perhaps reversible by immune-based therapies; we hope that, in the not too distant future, our studies will enable development of a vaccine to prevent CNS aging and cognitive loss in elderly.
Summary
With increased life expectancy, there has been a critical growth in the portion of the population that suffers from age-related cognitive decline and dementia. Attempts are therefore being made to find ways to slow brain-aging processes; successful therapies would have a significant impact on the quality of life of individuals, and decrease healthcare expenditures. Aging of the immune system has never been suggested as a factor in memory loss. My group formulated the concept of protective autoimmunity , suggesting a linkage between immunity and self-maintenance in the context of the brain in health and disease. Recently, we showed that T lymphocytes recognizing brain-self antigens have a pivotal role in maintaining hippocampal plasticity, as manifested by reduced neurogenesis and impaired cognitive abilities in T-cell deficient mice. Taken together, our novel observations that T cell immunity contributes to hippocampal plasticity, and the fact that T cell immunity decreases with progressive aging create the basis for the present proposal. We will focus on the following questions: (a) Which aspects of cognition are supported by the immune system- learning, memory or both; (b) whether aging of the immune system is sufficient to induce aging of the brain; (c) whether activation of the immune system is sufficient to reverse age-related cognitive decline; (d) the mechanism underlying the effect of peripheral immunity on brain cognition; and (e) potential therapeutic implications of our findings. Our preliminary results demonstrate that the immune system contributes to spatial memory, and that imposing an immune deficiency is sufficient to cause a reversible memory deficit. These findings give strong reason for optimism that memory loss in the elderly is preventable and perhaps reversible by immune-based therapies; we hope that, in the not too distant future, our studies will enable development of a vaccine to prevent CNS aging and cognitive loss in elderly.
Max ERC Funding
1 650 000 €
Duration
Start date: 2009-01-01, End date: 2012-12-31
Project acronym NANOSQUID
Project Scanning Nano-SQUID on a Tip
Researcher (PI) Eli Zeldov
Host Institution (HI) WEIZMANN INSTITUTE OF SCIENCE
Call Details Advanced Grant (AdG), PE3, ERC-2008-AdG
Summary At the boundaries of physics research it is constantly necessary to introduce new tools and methods to expand the horizons and address fundamental issues. In this proposal, we will develop and then apply radically new tools that will enable groundbreaking progress in the field of vortex matter in superconductors and will be of great importance to condensed matter physics and nanoscience. We propose a new scanning magnetic imaging method based on self-aligned fabrication of Josephson junctions with characteristic sizes of 10 nm and superconducting quantum interference devices (SQUID) with typical diameter of 100 nm on the end of a pulled quartz tip. Such nano-SQUID on a tip will provide high-sensitivity high-bandwidth mapping of static and dynamic magnetic fields on nanometer scale that is significantly beyond the state of the art. We will develop a new washboard frequency dynamic microscopy for imaging of site-dependent vortex velocities over a remarkable range of over six orders of magnitude in velocity that is expected to reveal the most interesting dynamic phenomena in vortex mater that could not be investigated so far. Our study will provide a novel bottom-up comprehension of microscopic vortex dynamics from single vortex up to numerous predicted dynamic phase transitions, including disorder-dependent depinning processes, plastic deformations, channel flow, metastabilities and memory effects, moving smectic, moving Bragg glass, and dynamic melting. We will also develop a hybrid technology that combines a single electron transistor with nano-SQUID which will provide an unprecedented simultaneous nanoscale imaging of magnetic and electric fields. Using these tools we will carry out innovative studies of additional nano-systems and exciting quantum phenomena, including quantum tunneling in molecular magnets, spin injection and magnetic domain wall dynamics, vortex charge, unconventional superconductivity, and coexistence of superconductivity and ferromagnetism.
Summary
At the boundaries of physics research it is constantly necessary to introduce new tools and methods to expand the horizons and address fundamental issues. In this proposal, we will develop and then apply radically new tools that will enable groundbreaking progress in the field of vortex matter in superconductors and will be of great importance to condensed matter physics and nanoscience. We propose a new scanning magnetic imaging method based on self-aligned fabrication of Josephson junctions with characteristic sizes of 10 nm and superconducting quantum interference devices (SQUID) with typical diameter of 100 nm on the end of a pulled quartz tip. Such nano-SQUID on a tip will provide high-sensitivity high-bandwidth mapping of static and dynamic magnetic fields on nanometer scale that is significantly beyond the state of the art. We will develop a new washboard frequency dynamic microscopy for imaging of site-dependent vortex velocities over a remarkable range of over six orders of magnitude in velocity that is expected to reveal the most interesting dynamic phenomena in vortex mater that could not be investigated so far. Our study will provide a novel bottom-up comprehension of microscopic vortex dynamics from single vortex up to numerous predicted dynamic phase transitions, including disorder-dependent depinning processes, plastic deformations, channel flow, metastabilities and memory effects, moving smectic, moving Bragg glass, and dynamic melting. We will also develop a hybrid technology that combines a single electron transistor with nano-SQUID which will provide an unprecedented simultaneous nanoscale imaging of magnetic and electric fields. Using these tools we will carry out innovative studies of additional nano-systems and exciting quantum phenomena, including quantum tunneling in molecular magnets, spin injection and magnetic domain wall dynamics, vortex charge, unconventional superconductivity, and coexistence of superconductivity and ferromagnetism.
Max ERC Funding
2 000 000 €
Duration
Start date: 2008-12-01, End date: 2013-11-30
Project acronym REGULATORYCIRCUITS
Project Novel Systematic Strategies for Elucidating Cellular Regulatory Circuits
Researcher (PI) Nir Friedman
Host Institution (HI) THE HEBREW UNIVERSITY OF JERUSALEM
Call Details Advanced Grant (AdG), LS2, ERC-2008-AdG
Summary The precise regulation of gene expression has been the subject of extensive scrutiny. Nonetheless, there is a big gap between genomic characterization of transcriptional responses and our predictions based on known molecular mechanisms and networks and of transcription regulation. In this proposal I argue for an approach to bridge this gap by using a novel experimental strategy that exploits the recent maturation of two technologies: the use of fluorescence reporter techniques to monitor promoter activity and high-throughput genetic manipulations for the construction of combinatorial genetic perturbations. By combining these, we will screen for genes that modulate the transcriptional response of target promoters, use genetic interactions between them to better resolve their functional dependencies, and build detailed quantitative models of transcriptional processes. We will use the budding yeast model organism, which allows for efficient manipulations, to dissect two transcriptional responses that are prototypical of many regulatory networks in living cells: [1] The early response to osmotic stress, which is mediated by at least two signaling pathways and multiple transcription factors, and [2] the central carbon metabolism response to shifts in carbon source, which involves multiple sensing and signaling pathways to maintain homeostasis. Our approach will elucidate mechanisms that are opaque to classical screens and facilitate building detailed predictive models of these responses. These results will lead to understanding of general principles that govern transcriptional networks. This is the first approach to comprehensively characterize the molecular mechanisms that modulate a transcriptional response, and arrange them in a coherent network. It will open many questions for detailed biochemical investigations, as well as set the stage to extend these ideas to use more detailed phenotypic assays and in more complex organisms.
Summary
The precise regulation of gene expression has been the subject of extensive scrutiny. Nonetheless, there is a big gap between genomic characterization of transcriptional responses and our predictions based on known molecular mechanisms and networks and of transcription regulation. In this proposal I argue for an approach to bridge this gap by using a novel experimental strategy that exploits the recent maturation of two technologies: the use of fluorescence reporter techniques to monitor promoter activity and high-throughput genetic manipulations for the construction of combinatorial genetic perturbations. By combining these, we will screen for genes that modulate the transcriptional response of target promoters, use genetic interactions between them to better resolve their functional dependencies, and build detailed quantitative models of transcriptional processes. We will use the budding yeast model organism, which allows for efficient manipulations, to dissect two transcriptional responses that are prototypical of many regulatory networks in living cells: [1] The early response to osmotic stress, which is mediated by at least two signaling pathways and multiple transcription factors, and [2] the central carbon metabolism response to shifts in carbon source, which involves multiple sensing and signaling pathways to maintain homeostasis. Our approach will elucidate mechanisms that are opaque to classical screens and facilitate building detailed predictive models of these responses. These results will lead to understanding of general principles that govern transcriptional networks. This is the first approach to comprehensively characterize the molecular mechanisms that modulate a transcriptional response, and arrange them in a coherent network. It will open many questions for detailed biochemical investigations, as well as set the stage to extend these ideas to use more detailed phenotypic assays and in more complex organisms.
Max ERC Funding
2 199 899 €
Duration
Start date: 2009-01-01, End date: 2013-12-31
Project acronym VARB
Project Variability and Robustness in Bio-molecular systems
Researcher (PI) Naama Barkai
Host Institution (HI) WEIZMANN INSTITUTE OF SCIENCE
Call Details Advanced Grant (AdG), LS2, ERC-2008-AdG
Summary Cells process information using biochemical networks of interacting proteins and genes. We wish to understand the principles that guide the design of such networks. In particular, we are interested in the interplay between variability, inherent to biological systems, and the precision of cellular computing. To better understand this interplay, we will: (1) Characterize the extent of gene expression variability and define its genetic determinants, (2) Reveal how variability is buffered and (3) Describe instances where variability (or 'noise') is an integral part of cellular computation. The study will be conducted in the multidisciplinary atmosphere of our lab, by students trained in physics, computer science, chemistry and biology. Specific issues include: 1. Gene expression variability: we will focus on the influence of chromatin structure on gene expression variability, as suggested by our bioinformatics analysis. 2. Robustness and scaling in embryonic patterning: We will study the means by which fluctuations are buffered during the development of multicellular organisms. We will focus on the robustness of morphogen gradients to protein levels, and on the ability to maintain proportionate pattern in tissues of different size. 3. Noise-driven transitions in a fluctuating environment: Our preliminary results suggest that noise plays an integral part in phosphate homeostasis in S. cerevisiae. We will characterize the role of noise in this system and study its evolutionary implications. Together, our study will shed light on one we believe to be the fundamental challenge of biological information processing: ensuring a reliable and reproducible function in the highly variable biological environment. Our study will furthermore define novel multidisciplinary, system-level paradigms and approaches that will guide further studies of bio-molecular systems
Summary
Cells process information using biochemical networks of interacting proteins and genes. We wish to understand the principles that guide the design of such networks. In particular, we are interested in the interplay between variability, inherent to biological systems, and the precision of cellular computing. To better understand this interplay, we will: (1) Characterize the extent of gene expression variability and define its genetic determinants, (2) Reveal how variability is buffered and (3) Describe instances where variability (or 'noise') is an integral part of cellular computation. The study will be conducted in the multidisciplinary atmosphere of our lab, by students trained in physics, computer science, chemistry and biology. Specific issues include: 1. Gene expression variability: we will focus on the influence of chromatin structure on gene expression variability, as suggested by our bioinformatics analysis. 2. Robustness and scaling in embryonic patterning: We will study the means by which fluctuations are buffered during the development of multicellular organisms. We will focus on the robustness of morphogen gradients to protein levels, and on the ability to maintain proportionate pattern in tissues of different size. 3. Noise-driven transitions in a fluctuating environment: Our preliminary results suggest that noise plays an integral part in phosphate homeostasis in S. cerevisiae. We will characterize the role of noise in this system and study its evolutionary implications. Together, our study will shed light on one we believe to be the fundamental challenge of biological information processing: ensuring a reliable and reproducible function in the highly variable biological environment. Our study will furthermore define novel multidisciplinary, system-level paradigms and approaches that will guide further studies of bio-molecular systems
Max ERC Funding
2 200 000 €
Duration
Start date: 2009-01-01, End date: 2013-10-31
