Project acronym APARTHEID-STOPS
Project Apartheid -- The Global Itinerary: South African Cultural Formations in Transnational Circulation, 1948-1990
Researcher (PI) Louise Bethlehem
Host Institution (HI) THE HEBREW UNIVERSITY OF JERUSALEM
Call Details Consolidator Grant (CoG), SH5, ERC-2013-CoG
Summary This proposal proceeds from an anomaly. Apartheid routinely breached the separation that it names. Whereas the South African regime was deeply isolationist in international terms, new research links it to the Cold War and decolonization. Yet this trend does not consider sufficiently that the global contest over the meaning of apartheid and resistance to it occurs on the terrain of culture. My project argues that studying the global circulation of South African cultural formations in the apartheid era provides novel historiographic leverage over Western liberalism during the Cold War. It recasts apartheid as an apparatus of transnational cultural production, turning existing historiography inside out. This study seeks:
• To provide the first systematic account of the deterritorialization of “apartheid”—as political signifier and as apparatus generating circuits of transnational cultural production.
• To analyze these itinerant cultural formations across media and national borders, articulating new intersections.
• To map the itineraries of major South African exiles, where exile is taken to be a system of interlinked circuits of affiliation and cultural production.
• To revise the historiography of states other than South Africa through the lens of deterritorialized apartheid-era formations at their respective destinations.
• To show how apartheid reveals contradictions within Western liberalism during the Cold War, with special reference to racial inequality.
Methodologically, I introduce the model of thick convergence to analyze three periods:
1. Kliptown & Bandung: Novel possibilities, 1948-1960.
2. Sharpeville & Memphis: Drumming up resistance, 1960-1976.
3. From Soweto to Berlin: Spectacle at the barricades, 1976-1990.
Each explores a cultural dominant in the form of texts, soundscapes or photographs. My work stands at the frontier of transnational research, furnishing powerful new insights into why South Africa matters on the stage of global history.
Summary
This proposal proceeds from an anomaly. Apartheid routinely breached the separation that it names. Whereas the South African regime was deeply isolationist in international terms, new research links it to the Cold War and decolonization. Yet this trend does not consider sufficiently that the global contest over the meaning of apartheid and resistance to it occurs on the terrain of culture. My project argues that studying the global circulation of South African cultural formations in the apartheid era provides novel historiographic leverage over Western liberalism during the Cold War. It recasts apartheid as an apparatus of transnational cultural production, turning existing historiography inside out. This study seeks:
• To provide the first systematic account of the deterritorialization of “apartheid”—as political signifier and as apparatus generating circuits of transnational cultural production.
• To analyze these itinerant cultural formations across media and national borders, articulating new intersections.
• To map the itineraries of major South African exiles, where exile is taken to be a system of interlinked circuits of affiliation and cultural production.
• To revise the historiography of states other than South Africa through the lens of deterritorialized apartheid-era formations at their respective destinations.
• To show how apartheid reveals contradictions within Western liberalism during the Cold War, with special reference to racial inequality.
Methodologically, I introduce the model of thick convergence to analyze three periods:
1. Kliptown & Bandung: Novel possibilities, 1948-1960.
2. Sharpeville & Memphis: Drumming up resistance, 1960-1976.
3. From Soweto to Berlin: Spectacle at the barricades, 1976-1990.
Each explores a cultural dominant in the form of texts, soundscapes or photographs. My work stands at the frontier of transnational research, furnishing powerful new insights into why South Africa matters on the stage of global history.
Max ERC Funding
1 861 238 €
Duration
Start date: 2014-05-01, End date: 2019-04-30
Project acronym Emotions in Conflict
Project Direct and Indirect Emotion Regulation as a New Path of Conflict Resolution
Researcher (PI) Eran Halperin
Host Institution (HI) INTERDISCIPLINARY CENTER (IDC) HERZLIYA
Call Details Starting Grant (StG), SH4, ERC-2013-StG
Summary Intractable conflicts are one of the gravest challenges to both humanity and science. These conflicts are initiated and perpetuated by people; therefore changing people's hearts and minds constitutes a huge step towards resolution. Research on emotions in conflicts has led to the realization that intergroup emotions are critical to conflict dynamics. This project’s intrinsic question is whether and how intergroup emotions can be regulated to alter attitudes and behavior towards peace. I offer an innovative path, using two strategies of emotion regulation. The first is Direct Emotion Regulation, where traditional, effective emotion regulation strategies can be used to change intergroup emotional experiences and subsequently political positions in conflict situations. The second, Indirect Emotion Regulation, serves to implicitly alter concrete cognitive appraisals, thus changing attitudes by changing discrete emotions. This is the first attempt ever to integrate psychological aggregated knowledge on emotion regulation with conflict resolution. I propose 16 studies, conducted in the context of the intractable Israeli-Palestinian conflict. Seven studies will focus on direct emotion regulation, reducing intergroup anger and hatred, while 9 studies will focus on indirect regulation, aspiring to reduce fear and despair. In both paths, correlational and in-lab experimental studies will be used to refine adequate strategies of down regulating destructive emotions, the results of which will be used to develop innovative, theory-driven education and media interventions that will be tested utilizing wide scale experience sampling methodology. This project aspires to bridge the gap between basic and applied science, creating a pioneering, interdisciplinary framework which contributes to existing knowledge on emotion regulation in conflict and implements ways to apply it in real-world circumstances.
Summary
Intractable conflicts are one of the gravest challenges to both humanity and science. These conflicts are initiated and perpetuated by people; therefore changing people's hearts and minds constitutes a huge step towards resolution. Research on emotions in conflicts has led to the realization that intergroup emotions are critical to conflict dynamics. This project’s intrinsic question is whether and how intergroup emotions can be regulated to alter attitudes and behavior towards peace. I offer an innovative path, using two strategies of emotion regulation. The first is Direct Emotion Regulation, where traditional, effective emotion regulation strategies can be used to change intergroup emotional experiences and subsequently political positions in conflict situations. The second, Indirect Emotion Regulation, serves to implicitly alter concrete cognitive appraisals, thus changing attitudes by changing discrete emotions. This is the first attempt ever to integrate psychological aggregated knowledge on emotion regulation with conflict resolution. I propose 16 studies, conducted in the context of the intractable Israeli-Palestinian conflict. Seven studies will focus on direct emotion regulation, reducing intergroup anger and hatred, while 9 studies will focus on indirect regulation, aspiring to reduce fear and despair. In both paths, correlational and in-lab experimental studies will be used to refine adequate strategies of down regulating destructive emotions, the results of which will be used to develop innovative, theory-driven education and media interventions that will be tested utilizing wide scale experience sampling methodology. This project aspires to bridge the gap between basic and applied science, creating a pioneering, interdisciplinary framework which contributes to existing knowledge on emotion regulation in conflict and implements ways to apply it in real-world circumstances.
Max ERC Funding
1 499 344 €
Duration
Start date: 2014-02-01, End date: 2019-01-31
Project acronym GRAMBY
Project The Grammar of the Body: Revealing the Foundations of Compositionality in Human Language
Researcher (PI) Wendy Sandler
Host Institution (HI) UNIVERSITY OF HAIFA
Call Details Advanced Grant (AdG), SH4, ERC-2013-ADG
Summary The pioneering framework I propose for the analysis of the foundations of human language – the Grammar of the Body – is inspired by sign language. My main aim is to create a body-based model of linguistic compositionality and to provide clues of its evolutionary origins.
Instead of analysing sign language (and language generally) from the perspective of mental categories, the radical approach I introduce here analyses language from the outside in, from the physical articulators of the face, hands, and body in sign language, to the grammatical structures they manifest. This new approach capitalizes on the discovery that gestures of each articulator make a meaningful contribution to the whole corporeal display, and yield a hierarchy from small to large in both body and grammar domains: hands/words > head and face/phrasal intonation > torso/discourse perspective. I hypothesize that the corporeal base of compositionality has deeper evolutionary roots in the emotional face and body displays of humans and our closest living relatives, chimpanzees.
The multi-disciplinary methodology I adopt will incorporate linguistic analysis of established and newly emerging sign languages with artistic manipulation of language form, and allow us to trace the origins of the system in emotional displays of both humans and apes.
My central goal – determining the basis and structure of compositionality in human language – and the unconventional methodological approaches it exploits combine to make this an extremely ambitious proposal with potentially wide-reaching ramifications in the humanities and social sciences.
Summary
The pioneering framework I propose for the analysis of the foundations of human language – the Grammar of the Body – is inspired by sign language. My main aim is to create a body-based model of linguistic compositionality and to provide clues of its evolutionary origins.
Instead of analysing sign language (and language generally) from the perspective of mental categories, the radical approach I introduce here analyses language from the outside in, from the physical articulators of the face, hands, and body in sign language, to the grammatical structures they manifest. This new approach capitalizes on the discovery that gestures of each articulator make a meaningful contribution to the whole corporeal display, and yield a hierarchy from small to large in both body and grammar domains: hands/words > head and face/phrasal intonation > torso/discourse perspective. I hypothesize that the corporeal base of compositionality has deeper evolutionary roots in the emotional face and body displays of humans and our closest living relatives, chimpanzees.
The multi-disciplinary methodology I adopt will incorporate linguistic analysis of established and newly emerging sign languages with artistic manipulation of language form, and allow us to trace the origins of the system in emotional displays of both humans and apes.
My central goal – determining the basis and structure of compositionality in human language – and the unconventional methodological approaches it exploits combine to make this an extremely ambitious proposal with potentially wide-reaching ramifications in the humanities and social sciences.
Max ERC Funding
2 448 318 €
Duration
Start date: 2014-07-01, End date: 2019-06-30
Project acronym HumanTrafficking
Project Human Trafficking: A Labor Perspective
Researcher (PI) Hila Shamir
Host Institution (HI) TEL AVIV UNIVERSITY
Call Details Starting Grant (StG), SH2, ERC-2017-STG
Summary This project conducts a theoretical, methodological, and normative paradigm shift in the research and analysis of human trafficking, one of the most pressing moral and political challenges of our times. It moves away from the currently predominant approach to trafficking, which focuses on criminal law, border control, and human rights, towards a labor-based approach that targets the structure of labor markets that are prone to severely exploitative labor practices. This shift represents an essential development both in the research of migratory labor practices and in the process of designing more effective, and more just, anti-trafficking measures, that are context-sensitive as well as cognizant to global legal and economic trends. The project will include four main parts: 1) Theoretical: articulating and justifying the proposed shift on trafficking from individual rights and culpabilities to structural labor market realities. 2) Case-studies: conducting a multidisciplinary study of a series of innovative case studies, in which the labor context emerges as a significant factor in the trafficking nexus – bilateral agreements on migration, national regulations of labor standards and recruiters, unionization, and voluntary corporate codes of conduct. The case studies analysis employs the labor paradigm in elucidating the structural conditions that underlie trafficking, reveal a thus-far mostly unrecognized and under-theorized set of anti-trafficking tools. 3) Clinical Laboratory: collaborating with TAUs Workers' Rights clinic to create a legal laboratory in which the potential and limits of the tools examined in the case studies will be tested. 4) Normative: assessing the success of existing strategies and expanding on them to devise innovative tools for a just, practicable, and effective anti-trafficking policy, that can reach significantly more individuals vulnerable to trafficking, by providing them with legal mechanisms for avoiding and resisting exploitation.
Summary
This project conducts a theoretical, methodological, and normative paradigm shift in the research and analysis of human trafficking, one of the most pressing moral and political challenges of our times. It moves away from the currently predominant approach to trafficking, which focuses on criminal law, border control, and human rights, towards a labor-based approach that targets the structure of labor markets that are prone to severely exploitative labor practices. This shift represents an essential development both in the research of migratory labor practices and in the process of designing more effective, and more just, anti-trafficking measures, that are context-sensitive as well as cognizant to global legal and economic trends. The project will include four main parts: 1) Theoretical: articulating and justifying the proposed shift on trafficking from individual rights and culpabilities to structural labor market realities. 2) Case-studies: conducting a multidisciplinary study of a series of innovative case studies, in which the labor context emerges as a significant factor in the trafficking nexus – bilateral agreements on migration, national regulations of labor standards and recruiters, unionization, and voluntary corporate codes of conduct. The case studies analysis employs the labor paradigm in elucidating the structural conditions that underlie trafficking, reveal a thus-far mostly unrecognized and under-theorized set of anti-trafficking tools. 3) Clinical Laboratory: collaborating with TAUs Workers' Rights clinic to create a legal laboratory in which the potential and limits of the tools examined in the case studies will be tested. 4) Normative: assessing the success of existing strategies and expanding on them to devise innovative tools for a just, practicable, and effective anti-trafficking policy, that can reach significantly more individuals vulnerable to trafficking, by providing them with legal mechanisms for avoiding and resisting exploitation.
Max ERC Funding
1 492 250 €
Duration
Start date: 2018-04-01, End date: 2023-03-31
Project acronym INTEGRATION
Project International Integration and Social Identity: Theory and Evidence
Researcher (PI) Moses Shayo
Host Institution (HI) THE HEBREW UNIVERSITY OF JERUSALEM
Call Details Starting Grant (StG), SH1, ERC-2013-StG
Summary Understanding economic and political integration has long been a central concern for economists. An important missing ingredient in the existing literature is the analysis of endogenously determined social identity. By “social identity” I refer to the fact that individuals often care deeply about the groups to which they belong. By “endogenously determined” I refer to the fact that individuals do not automatically identify with every group they belong to: whether or not an individual identifies with a given group depends on the characteristics of this group as well as on how close to this group the individual perceives herself. Empirical results obtained over the past decade allow us to integrate identity concerns into standard economic models. I propose to develop and test a theory of integration that does just that.
Consider two states that may either be independent countries or form a union. The stability and desirability of unification may sometimes depend on the extent to which citizens identify with the union or with their states. But the profile of identities itself depends on the political-economic outcome under unification. The first step in developing the theory is to translate the evidence concerning behavior in groups into a concise statement of what it means to “identify” with a particular group and what factors shape identification decisions. The theory will then study the equilibrium outcomes of a political economy model of integration, where actions and identities are endogenously determined.
The second part of the project will empirically examine the relation between social identities, individual characteristics, and European integration. To appropriately measure identification, I propose to employ experimental methods based on revealed preference conducted with a large and diverse sample of European citizens. This will be complemented by historical multi-country survey data on self-reported identity, political attitudes and behavior.
Summary
Understanding economic and political integration has long been a central concern for economists. An important missing ingredient in the existing literature is the analysis of endogenously determined social identity. By “social identity” I refer to the fact that individuals often care deeply about the groups to which they belong. By “endogenously determined” I refer to the fact that individuals do not automatically identify with every group they belong to: whether or not an individual identifies with a given group depends on the characteristics of this group as well as on how close to this group the individual perceives herself. Empirical results obtained over the past decade allow us to integrate identity concerns into standard economic models. I propose to develop and test a theory of integration that does just that.
Consider two states that may either be independent countries or form a union. The stability and desirability of unification may sometimes depend on the extent to which citizens identify with the union or with their states. But the profile of identities itself depends on the political-economic outcome under unification. The first step in developing the theory is to translate the evidence concerning behavior in groups into a concise statement of what it means to “identify” with a particular group and what factors shape identification decisions. The theory will then study the equilibrium outcomes of a political economy model of integration, where actions and identities are endogenously determined.
The second part of the project will empirically examine the relation between social identities, individual characteristics, and European integration. To appropriately measure identification, I propose to employ experimental methods based on revealed preference conducted with a large and diverse sample of European citizens. This will be complemented by historical multi-country survey data on self-reported identity, political attitudes and behavior.
Max ERC Funding
1 050 000 €
Duration
Start date: 2014-03-01, End date: 2019-02-28
Project acronym JUDGINGHISTORIES
Project Experience, Judgement, and Representation of World War II in an Age of Globalization
Researcher (PI) Dan Diner
Host Institution (HI) THE HEBREW UNIVERSITY OF JERUSALEM
Call Details Advanced Grant (AdG), SH6, ERC-2013-ADG
Summary “JudgingHistories” sets out to examine the epistemic premises innate to universalizing historical experience, by scrutinizing the quest for historical understanding and moral judgment against the backdrop of an emerging global cultural environment, fraught with multiple recollections, while using memories of World War II as the empirical core of the study. The pivotal constellation of research emerges by interfacing a horizontal (West-East) alignment traditionally significant for continental European history with a vertically oriented alignment (North-South) that sheds a colonial and post-colonial perspective on World War II. This constellation tends to lead a posteriori to a realm of conflicting, morally permeated discourses of comparison and analogy, revealing the Holocaust to function as the central event of continental narration, on the one hand, while genocidal atrocities highlight the colonial or post-colonial comprehension, perception and narration, on the other. Methodologically, and in order to offer a fresh and innovative view of the emergence of the specifics of knowledge and meaning in the domain of historical understanding in a globalizing world, while placing the signifying event of the Nazis’ systematic annihilation of the Jews at the heart of the question of universal historical judgment, the project proceeds from the colonial periphery of events, however. This “peripheral”, colonial perspective will in a further seemingly paradoxical turn find itself extended into continental European affairs where it functions to help us comprehend the multiplicity of experiences and the diversity of attendant memories unfolding there. Such a research perspective may epistemologically enable us to reconstruct a universally convincing and valid understanding of a foundational event in European and global history, namely the recollection of World War II, and thus render possible common judgment while re-determining the meaning of “History”.
Summary
“JudgingHistories” sets out to examine the epistemic premises innate to universalizing historical experience, by scrutinizing the quest for historical understanding and moral judgment against the backdrop of an emerging global cultural environment, fraught with multiple recollections, while using memories of World War II as the empirical core of the study. The pivotal constellation of research emerges by interfacing a horizontal (West-East) alignment traditionally significant for continental European history with a vertically oriented alignment (North-South) that sheds a colonial and post-colonial perspective on World War II. This constellation tends to lead a posteriori to a realm of conflicting, morally permeated discourses of comparison and analogy, revealing the Holocaust to function as the central event of continental narration, on the one hand, while genocidal atrocities highlight the colonial or post-colonial comprehension, perception and narration, on the other. Methodologically, and in order to offer a fresh and innovative view of the emergence of the specifics of knowledge and meaning in the domain of historical understanding in a globalizing world, while placing the signifying event of the Nazis’ systematic annihilation of the Jews at the heart of the question of universal historical judgment, the project proceeds from the colonial periphery of events, however. This “peripheral”, colonial perspective will in a further seemingly paradoxical turn find itself extended into continental European affairs where it functions to help us comprehend the multiplicity of experiences and the diversity of attendant memories unfolding there. Such a research perspective may epistemologically enable us to reconstruct a universally convincing and valid understanding of a foundational event in European and global history, namely the recollection of World War II, and thus render possible common judgment while re-determining the meaning of “History”.
Max ERC Funding
2 499 260 €
Duration
Start date: 2014-06-01, End date: 2019-05-31
Project acronym NEEM
Project The New Ecology of Expressive Modes in Early Modern South India
Researcher (PI) David Dean Shulman
Host Institution (HI) THE HEBREW UNIVERSITY OF JERUSALEM
Call Details Advanced Grant (AdG), SH5, ERC-2017-ADG
Summary Southern India in the fifteenth, sixteenth, and seventeenth centuries comprised a single, strongly interwoven, multilingual cultural world that generated innovative literary, musical, theatrical and visual masterpieces as well as a theoretical erudite literature that explored the aesthetic and philosophical bases of these new works. New literary genres such as the compact, self-contained prabandha narratives crossed linguistic boundaries, emerging in and rapidly coming to dominate Tamil, Telugu, Kannada, Malayalam, and the trans-local languages of Sanskrit, Persian, Marathi and Dakhni. Such texts, seen together with major genres evolving in the other expressive domains (the early varnams and kirttanas in music, the great mural paintings of the Tamil and Karnataka regions, Kudiyattam drama), were building blocks of an eco-system whose rules, themes, forms, and intertextual relations have never been studied as a whole. We propose to explore this large corpus in relation to the new grammars of language, poetics, music, drama, and painting that evolved at the same time. Among major themes common to all these traditions are the interiority and states of mind of the individual human person, the question of how such a person is created and fashioned, the problem of the fate or destiny that a person faces or generates out of himself or herself, and the autonomy of the natural world within which he or she lives and acts. All the major expressive domains, with their thematic and theoretical continuities, give voice to a historic shift in the dynamics and underlying axioms of south Indian civilization at the start of the modern age; this shift becomes fully articulate and apparent only when we see the expressive eco-system as a whole.
Summary
Southern India in the fifteenth, sixteenth, and seventeenth centuries comprised a single, strongly interwoven, multilingual cultural world that generated innovative literary, musical, theatrical and visual masterpieces as well as a theoretical erudite literature that explored the aesthetic and philosophical bases of these new works. New literary genres such as the compact, self-contained prabandha narratives crossed linguistic boundaries, emerging in and rapidly coming to dominate Tamil, Telugu, Kannada, Malayalam, and the trans-local languages of Sanskrit, Persian, Marathi and Dakhni. Such texts, seen together with major genres evolving in the other expressive domains (the early varnams and kirttanas in music, the great mural paintings of the Tamil and Karnataka regions, Kudiyattam drama), were building blocks of an eco-system whose rules, themes, forms, and intertextual relations have never been studied as a whole. We propose to explore this large corpus in relation to the new grammars of language, poetics, music, drama, and painting that evolved at the same time. Among major themes common to all these traditions are the interiority and states of mind of the individual human person, the question of how such a person is created and fashioned, the problem of the fate or destiny that a person faces or generates out of himself or herself, and the autonomy of the natural world within which he or she lives and acts. All the major expressive domains, with their thematic and theoretical continuities, give voice to a historic shift in the dynamics and underlying axioms of south Indian civilization at the start of the modern age; this shift becomes fully articulate and apparent only when we see the expressive eco-system as a whole.
Max ERC Funding
2 009 153 €
Duration
Start date: 2018-10-01, End date: 2023-09-30
Project acronym NEXTGENBIM
Project NEXT-GENERATION BUILDING INFORMATION MODELING TO SUPPORT EVALUATION OF HUMAN BEHAVIOR IN BUILT ENVIRONMENTS
Researcher (PI) Yehuda Kalay
Host Institution (HI) TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY
Call Details Advanced Grant (AdG), SH3, ERC-2013-ADG
Summary This proposal argues that current building modeling tools, including popular BIM (Building Information Modeling) systems, provide a poor, inadequate representation of buildings: they represent only the physical and material characteristics of buildings. Buildings, unlike other products, cannot be understood independently of their context, of their intended use, and of their intended users.
This shortcoming hinders the ability of current building models to support evaluations other than those based on physical and material characteristics of the building, such as lighting, energy consumption, and structural stability. In particular, the impact of a building that has not yet been built on the life and activities of its future users—a key element in determining whether or not the proposed building will meet the needs of its intended users—is not afforded by current building models. To afford comprehensive prediction and evaluation of future buildings, we also need to model the purpose and function of the building, and the social, cultural, and economic profile of the people who will use it.
Although predicting users' behavior in a built environment and their interaction with the building and with other people is a highly complex task, vast research exists that is devoted to analyzing and explaining human behavior in built environments. Still, due to the shortcomings of building models, this knowledge rarely make into the practice of architectural design, at the time buildings are being designed.
The proposed research aims at remedying that shortcoming by developing a more a comprehensive building modeling method, which will include form, function, and use information. A better model will lead to better designed buildings. In an era when the irrevocable impact of the built environment on the cost, quality, and perhaps even possibility of life on earth has been recognized, the need to make every effort to improve the tools used by building designers is self-evident.
Summary
This proposal argues that current building modeling tools, including popular BIM (Building Information Modeling) systems, provide a poor, inadequate representation of buildings: they represent only the physical and material characteristics of buildings. Buildings, unlike other products, cannot be understood independently of their context, of their intended use, and of their intended users.
This shortcoming hinders the ability of current building models to support evaluations other than those based on physical and material characteristics of the building, such as lighting, energy consumption, and structural stability. In particular, the impact of a building that has not yet been built on the life and activities of its future users—a key element in determining whether or not the proposed building will meet the needs of its intended users—is not afforded by current building models. To afford comprehensive prediction and evaluation of future buildings, we also need to model the purpose and function of the building, and the social, cultural, and economic profile of the people who will use it.
Although predicting users' behavior in a built environment and their interaction with the building and with other people is a highly complex task, vast research exists that is devoted to analyzing and explaining human behavior in built environments. Still, due to the shortcomings of building models, this knowledge rarely make into the practice of architectural design, at the time buildings are being designed.
The proposed research aims at remedying that shortcoming by developing a more a comprehensive building modeling method, which will include form, function, and use information. A better model will lead to better designed buildings. In an era when the irrevocable impact of the built environment on the cost, quality, and perhaps even possibility of life on earth has been recognized, the need to make every effort to improve the tools used by building designers is self-evident.
Max ERC Funding
1 629 370 €
Duration
Start date: 2013-12-01, End date: 2018-11-30
Project acronym PolControl
Project Engineering translation machinery to produce light-responsive protein-polymers
Researcher (PI) Miriam AMIRAM
Host Institution (HI) BEN-GURION UNIVERSITY OF THE NEGEV
Call Details Starting Grant (StG), LS9, ERC-2017-STG
Summary A general and versatile technology to engineer visible light-responsive biological agents will enable spatio-temporal manipulation and interrogation of proteins, pathways, and cells, and the design of “smart” biomaterials that can direct and respond to biological processes on-demand. Site specific incorporation of multiple visible-light-responsive chemical groups at the polypeptide level will constitute a universal methodology for precise production of light-responsive proteins and protein-based materials. However, inadequate engineering of the protein translation apparatus limits the number and complexity of chemical groups that can be incorporated into proteins as synthetic amino acids (sAAs). This limitation precludes the incorporation of recently discovered visible-light-responsive chemical groups, hinders protein engineering efforts, and excludes production of biomaterials in which multiple identical sAAs provide new physical or biophysical properties. We propose to overcome this challenge by generating a genomic-engineering based platform for co-evolution of multiple components of the translation machinery (the aminoacyl tRNA synthetase, tRNA, and elongation factor) to select for cellular machinery capable of multi-site incorporation of highly substituted azobenzenes with a range of biologically relevant photochemical properties. We will then utilize these translation systems to produce libraries of azobenzene-containing protein-based materials to elucidate the sequence-function requirements for directing light-responsive self-assembly of macromolecular structures, and to generate biomaterial formulations for control of various intra- and extra-cellular processes. By developing and marrying technologies in synthetic biology, chemistry, and biomaterials, this study will enable the synthesis of light-responsive proteins, deepen our understanding of natural and evolved translation systems, and create new classes of functional light-responsive biomaterials.
Summary
A general and versatile technology to engineer visible light-responsive biological agents will enable spatio-temporal manipulation and interrogation of proteins, pathways, and cells, and the design of “smart” biomaterials that can direct and respond to biological processes on-demand. Site specific incorporation of multiple visible-light-responsive chemical groups at the polypeptide level will constitute a universal methodology for precise production of light-responsive proteins and protein-based materials. However, inadequate engineering of the protein translation apparatus limits the number and complexity of chemical groups that can be incorporated into proteins as synthetic amino acids (sAAs). This limitation precludes the incorporation of recently discovered visible-light-responsive chemical groups, hinders protein engineering efforts, and excludes production of biomaterials in which multiple identical sAAs provide new physical or biophysical properties. We propose to overcome this challenge by generating a genomic-engineering based platform for co-evolution of multiple components of the translation machinery (the aminoacyl tRNA synthetase, tRNA, and elongation factor) to select for cellular machinery capable of multi-site incorporation of highly substituted azobenzenes with a range of biologically relevant photochemical properties. We will then utilize these translation systems to produce libraries of azobenzene-containing protein-based materials to elucidate the sequence-function requirements for directing light-responsive self-assembly of macromolecular structures, and to generate biomaterial formulations for control of various intra- and extra-cellular processes. By developing and marrying technologies in synthetic biology, chemistry, and biomaterials, this study will enable the synthesis of light-responsive proteins, deepen our understanding of natural and evolved translation systems, and create new classes of functional light-responsive biomaterials.
Max ERC Funding
1 328 712 €
Duration
Start date: 2017-11-01, End date: 2022-10-31
Project acronym PS3
Project An artificial water-soluble photosystem by protein design
Researcher (PI) Dror Noy
Host Institution (HI) MIGAL GALILEE RESEARCH INSTITUTE LTD
Call Details Consolidator Grant (CoG), LS9, ERC-2013-CoG
Summary "This project aims at producing a fully functional light energy conversion system that is inspired by, but does not necessarily mimic, the fundamental solar energy conversion unit of natural photosynthesis – the photosystem. This is a formidable challenge that can be met with thorough understanding of biological energy and electron transfer processes, and the growing capabilities of computational protein design. Here, this knowledge and capabilities will be further developed and utilized for the design and construction of multi-cofactor, multi-subunit protein complexes with photosystem functionality. These will be designed to efficiently capture light in the visible and near infrared range, exploit it for driving the oxidation of a molecular redox carrier at one end, and providing highly reducing electrons at the other end.
Our general goal will be achieved by designing protein-cofactor complexes that will facilitate light-driven electron- and excitation energy-transfer that will make up the reaction center, and light harvesting modules, respectively. Constructing protein scaffolds that will assemble and organize arrays of multiple pigments, and chains of redox cofactors are significant challenges at the forefront of the field of protein de novo design, and current theories of biological energy and electron transfer.
Success will set a new standard, well beyond the current state of the art, for our ability to use computational protein design methods for assembling functional protein-cofactor complexes. These can be used as benchmarks to test and validate the engineering principles of biological energy conversion systems, as well as new ideas about their evolution. Practically, it will open new and exciting technological possibilities for constructing artificial solar energy conversion systems from biological building blocks, which may enable their introduction into living systems and the construction of novel bioreactors for light driven fuel production."
Summary
"This project aims at producing a fully functional light energy conversion system that is inspired by, but does not necessarily mimic, the fundamental solar energy conversion unit of natural photosynthesis – the photosystem. This is a formidable challenge that can be met with thorough understanding of biological energy and electron transfer processes, and the growing capabilities of computational protein design. Here, this knowledge and capabilities will be further developed and utilized for the design and construction of multi-cofactor, multi-subunit protein complexes with photosystem functionality. These will be designed to efficiently capture light in the visible and near infrared range, exploit it for driving the oxidation of a molecular redox carrier at one end, and providing highly reducing electrons at the other end.
Our general goal will be achieved by designing protein-cofactor complexes that will facilitate light-driven electron- and excitation energy-transfer that will make up the reaction center, and light harvesting modules, respectively. Constructing protein scaffolds that will assemble and organize arrays of multiple pigments, and chains of redox cofactors are significant challenges at the forefront of the field of protein de novo design, and current theories of biological energy and electron transfer.
Success will set a new standard, well beyond the current state of the art, for our ability to use computational protein design methods for assembling functional protein-cofactor complexes. These can be used as benchmarks to test and validate the engineering principles of biological energy conversion systems, as well as new ideas about their evolution. Practically, it will open new and exciting technological possibilities for constructing artificial solar energy conversion systems from biological building blocks, which may enable their introduction into living systems and the construction of novel bioreactors for light driven fuel production."
Max ERC Funding
1 997 944 €
Duration
Start date: 2014-06-01, End date: 2019-05-31