Project acronym AGRISCENTS
Project Scents and sensibility in agriculture: exploiting specificity in herbivore- and pathogen-induced plant volatiles for real-time crop monitoring
Researcher (PI) Theodoor Turlings
Host Institution (HI) UNIVERSITE DE NEUCHATEL
Country Switzerland
Call Details Advanced Grant (AdG), LS9, ERC-2017-ADG
Summary Plants typically release large quantities of volatiles in response to attack by herbivores or pathogens. I may claim to have contributed to various breakthroughs in this research field, including the discovery that the volatile blends induced by different attackers are astonishingly specific, resulting in characteristic, readily distinguishable odour blends. Using maize as our model plant, I wish to take several leaps forward in our understanding of this signal specificity and use this knowledge to develop sensors for the real-time detection of crop pests and diseases. For this, three interconnected work-packages will aim to:
• Develop chemical analytical techniques and statistical models to decipher the odorous vocabulary of plants, and to create a complete inventory of “odour-prints” for a wide range of herbivore-plant and pathogen-plant combinations, including simultaneous infestations.
• Develop and optimize nano-mechanical sensors for the detection of specific plant volatile mixtures. For this, we will initially adapt a prototype sensor that has been successfully developed for the detection of cancer-related volatiles in human breath.
• Genetically manipulate maize plants to release a unique blend of root-produced volatiles upon herbivory. For this, we will engineer gene cassettes that combine recently identified P450 (CYP) genes from poplar with inducible, root-specific promoters from maize. This will result in maize plants that, in response to pest attack, release easy-to-detect aldoximes and nitriles from their roots.
In short, by investigating and manipulating the specificity of inducible odour blends we will generate the necessary knowhow to develop a novel odour-detection device. The envisioned sensor technology will permit real-time monitoring of the pests and enable farmers to apply crop protection treatments at the right time and in the right place.
Summary
Plants typically release large quantities of volatiles in response to attack by herbivores or pathogens. I may claim to have contributed to various breakthroughs in this research field, including the discovery that the volatile blends induced by different attackers are astonishingly specific, resulting in characteristic, readily distinguishable odour blends. Using maize as our model plant, I wish to take several leaps forward in our understanding of this signal specificity and use this knowledge to develop sensors for the real-time detection of crop pests and diseases. For this, three interconnected work-packages will aim to:
• Develop chemical analytical techniques and statistical models to decipher the odorous vocabulary of plants, and to create a complete inventory of “odour-prints” for a wide range of herbivore-plant and pathogen-plant combinations, including simultaneous infestations.
• Develop and optimize nano-mechanical sensors for the detection of specific plant volatile mixtures. For this, we will initially adapt a prototype sensor that has been successfully developed for the detection of cancer-related volatiles in human breath.
• Genetically manipulate maize plants to release a unique blend of root-produced volatiles upon herbivory. For this, we will engineer gene cassettes that combine recently identified P450 (CYP) genes from poplar with inducible, root-specific promoters from maize. This will result in maize plants that, in response to pest attack, release easy-to-detect aldoximes and nitriles from their roots.
In short, by investigating and manipulating the specificity of inducible odour blends we will generate the necessary knowhow to develop a novel odour-detection device. The envisioned sensor technology will permit real-time monitoring of the pests and enable farmers to apply crop protection treatments at the right time and in the right place.
Max ERC Funding
2 498 086 €
Duration
Start date: 2018-09-01, End date: 2023-08-31
Project acronym AMIMOS
Project Agile MIMO Systems for Communications, Biomedicine, and Defense
Researcher (PI) Bjorn Ottersten
Host Institution (HI) KUNGLIGA TEKNISKA HOEGSKOLAN
Country Sweden
Call Details Advanced Grant (AdG), PE7, ERC-2008-AdG
Summary This proposal targets the emerging frontier research field of multiple-input multiple-output (MIMO) systems along with several innovative and somewhat unconventional applications of such systems. The use of arrays of transmitters and receivers will have a profound impact on future medical imaging/therapy systems, radar systems, and radio communication networks. Multiple transmitters provide a tremendous versatility and allow waveforms to be adapted temporally and spatially to environmental conditions. This is useful for individually tailored illumination of human tissue in biomedical imaging or ultrasound therapy. In radar systems, multiple transmit beams can be formed simultaneously via separate waveform designs allowing accurate target classification. In a wireless communication system, multiple communication signals can be directed to one or more users at the same time on the same frequency carrier. In addition, multiple receivers can be used in the above applications to provide increased detection performance, interference rejection, and improved estimation accuracy. The joint modelling, analysis, and design of these multidimensional transmit and receive schemes form the core of this research proposal. Ultimately, our research aims at developing the fundamental tools that will allow the design of wireless communication systems with an order-of-magnitude higher capacity at a lower cost than today; of ultrasound therapy systems maximizing delivered power while reducing treatment duration and unwanted illumination; and of distributed aperture multi-beam radars allowing more effective target location, identification, and classification. Europe has several successful industries that are active in biomedical imaging/therapy, radar systems, and wireless communications. The future success of these sectors critically depends on the ability to innovate and integrate new technology.
Summary
This proposal targets the emerging frontier research field of multiple-input multiple-output (MIMO) systems along with several innovative and somewhat unconventional applications of such systems. The use of arrays of transmitters and receivers will have a profound impact on future medical imaging/therapy systems, radar systems, and radio communication networks. Multiple transmitters provide a tremendous versatility and allow waveforms to be adapted temporally and spatially to environmental conditions. This is useful for individually tailored illumination of human tissue in biomedical imaging or ultrasound therapy. In radar systems, multiple transmit beams can be formed simultaneously via separate waveform designs allowing accurate target classification. In a wireless communication system, multiple communication signals can be directed to one or more users at the same time on the same frequency carrier. In addition, multiple receivers can be used in the above applications to provide increased detection performance, interference rejection, and improved estimation accuracy. The joint modelling, analysis, and design of these multidimensional transmit and receive schemes form the core of this research proposal. Ultimately, our research aims at developing the fundamental tools that will allow the design of wireless communication systems with an order-of-magnitude higher capacity at a lower cost than today; of ultrasound therapy systems maximizing delivered power while reducing treatment duration and unwanted illumination; and of distributed aperture multi-beam radars allowing more effective target location, identification, and classification. Europe has several successful industries that are active in biomedical imaging/therapy, radar systems, and wireless communications. The future success of these sectors critically depends on the ability to innovate and integrate new technology.
Max ERC Funding
1 872 720 €
Duration
Start date: 2009-01-01, End date: 2013-12-31
Project acronym ANALYTICAL SOCIOLOGY
Project Analytical Sociology: Theoretical Developments and Empirical Research
Researcher (PI) Mats Peter Hedstroem
Host Institution (HI) LINKOPINGS UNIVERSITET
Country Sweden
Call Details Advanced Grant (AdG), SH2, ERC-2012-ADG_20120411
Summary This proposal outlines a highly ambitious and path-breaking research program. Through a tightly integrated package of basic theoretical work, strategic empirical research projects, international workshops, and a large number of publications in leading journals, the research program seeks to move sociology in a more analytical direction.
One part of the research program focuses on the epistemological and methodological foundations of analytical sociology, an approach to sociological theory and research that currently receives considerable attention in the international scholarly community. This work will be organized around two core themes: (1) the principles of mechanism-based explanations and (2) the micro-macro link.
The empirical research analyzes in great detail the ethnic, gender, and socio-economic segregation of key interaction domains in Sweden using the approach of analytical sociology. The interaction domains focused upon are schools, workplaces and neighborhoods; domains where people spend a considerable part of their time, where much of the social interaction between people takes place, where identities are formed, and where important resources are distributed.
Large-scale longitudinal micro data on the entire Swedish population, unique longitudinal data on social networks within school classes, and various agent-based simulation techniques, are used to better understand the processes through which schools, workplaces and neighborhoods become segregated along various dimensions, how the domains interact with one another, and how the structure and extent of segregation affects diverse social and economic outcomes.
Summary
This proposal outlines a highly ambitious and path-breaking research program. Through a tightly integrated package of basic theoretical work, strategic empirical research projects, international workshops, and a large number of publications in leading journals, the research program seeks to move sociology in a more analytical direction.
One part of the research program focuses on the epistemological and methodological foundations of analytical sociology, an approach to sociological theory and research that currently receives considerable attention in the international scholarly community. This work will be organized around two core themes: (1) the principles of mechanism-based explanations and (2) the micro-macro link.
The empirical research analyzes in great detail the ethnic, gender, and socio-economic segregation of key interaction domains in Sweden using the approach of analytical sociology. The interaction domains focused upon are schools, workplaces and neighborhoods; domains where people spend a considerable part of their time, where much of the social interaction between people takes place, where identities are formed, and where important resources are distributed.
Large-scale longitudinal micro data on the entire Swedish population, unique longitudinal data on social networks within school classes, and various agent-based simulation techniques, are used to better understand the processes through which schools, workplaces and neighborhoods become segregated along various dimensions, how the domains interact with one another, and how the structure and extent of segregation affects diverse social and economic outcomes.
Max ERC Funding
1 745 098 €
Duration
Start date: 2013-03-01, End date: 2018-02-28
Project acronym BIOGEOS
Project Bio-mediated Geo-material Strengthening for engineering applications
Researcher (PI) Lyesse LALOUI
Host Institution (HI) ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE
Country Switzerland
Call Details Advanced Grant (AdG), PE8, ERC-2017-ADG
Summary Given the increasing scarcity of suitable land for development, soil strengthening technologies have emerged in the past decade and go hand-in-hand with the implementation of the majority of foundation solutions. The goal is to alter the soil structure and its mechanical properties for ultimately securing the integrity of structures. The BIOGEOS project puts the focus on bio-mediated soil improvement, which falls within the broader framework of multi-physical processes in geo-mechanics. The goal of the project is to engineer a novel, natural material under controlled processes, for ultimately providing solutions to real problems in the geo-engineering and geo-energy fields by advancing knowledge around complex multi-physical phenomena in porous media. The bio-cemented geo-material, which is produced by carefully integrating the metabolic activity of native soil bacteria, is produced through the bio-mineralization of calcite bonds, which act as natural cementation for endowing the subsurface with real cohesion and increased resistance. A principal characteristic of the project is its multi-scale approach through advanced experimentation to identify the main physical mechanisms involved in the formation of the bio-mineralized bonds and their behaviour under mechanical loading. The development of such a bio-mediated technology will lead to innovative applications in a series of engineering problems such as the restoration of weak foundations, seismic retrofitting, erosion protection, and the enhancement of heat transfer in thermo-active geo-structures. The project foresees to adopt multiple loading conditions for its laboratory characterization and ultimately pass to the large experimental scale. BIOGEOS further aims to provide new knowledge around the way we perceive materials in relation with their micro-structure by implementing state-of-the-art inspection of the material’s structure in 3D space and subsequent prediction of their behaviour through numerical tools.
Summary
Given the increasing scarcity of suitable land for development, soil strengthening technologies have emerged in the past decade and go hand-in-hand with the implementation of the majority of foundation solutions. The goal is to alter the soil structure and its mechanical properties for ultimately securing the integrity of structures. The BIOGEOS project puts the focus on bio-mediated soil improvement, which falls within the broader framework of multi-physical processes in geo-mechanics. The goal of the project is to engineer a novel, natural material under controlled processes, for ultimately providing solutions to real problems in the geo-engineering and geo-energy fields by advancing knowledge around complex multi-physical phenomena in porous media. The bio-cemented geo-material, which is produced by carefully integrating the metabolic activity of native soil bacteria, is produced through the bio-mineralization of calcite bonds, which act as natural cementation for endowing the subsurface with real cohesion and increased resistance. A principal characteristic of the project is its multi-scale approach through advanced experimentation to identify the main physical mechanisms involved in the formation of the bio-mineralized bonds and their behaviour under mechanical loading. The development of such a bio-mediated technology will lead to innovative applications in a series of engineering problems such as the restoration of weak foundations, seismic retrofitting, erosion protection, and the enhancement of heat transfer in thermo-active geo-structures. The project foresees to adopt multiple loading conditions for its laboratory characterization and ultimately pass to the large experimental scale. BIOGEOS further aims to provide new knowledge around the way we perceive materials in relation with their micro-structure by implementing state-of-the-art inspection of the material’s structure in 3D space and subsequent prediction of their behaviour through numerical tools.
Max ERC Funding
2 497 115 €
Duration
Start date: 2018-11-01, End date: 2024-04-30
Project acronym BIRD
Project Bimanual Manipulation of Rigid and Deformable Objects
Researcher (PI) Danica KRAGIC JENSFELT
Host Institution (HI) KUNGLIGA TEKNISKA HOEGSKOLAN
Country Sweden
Call Details Advanced Grant (AdG), PE7, ERC-2019-ADG
Summary All day long, our fingers touch, grasp and move objects in various media such as air, water, oil. We do this almost effortlessly - it feels like we do not spend time planning and reflecting over what our hands and fingers do or how the continuous integration of various sensory modalities such as vision, touch, proprioception, hearing help us to outperform any other biological system in the variety of the interaction tasks that we can execute. Largely overlooked, and perhaps most fascinating is the ease with which we perform these interactions resulting in a belief that these are also easy to accomplish in artificial systems such as robots. However, there are still no robots that can easily hand-wash dishes, button a shirt or peel a potato. Our claim is that this is fundamentally a problem of appropriate representation or parameterization. When interacting with objects, the robot needs to consider geometric, topological, and physical properties of objects. This can be done either explicitly, by modeling and representing these properties, or implicitly, by learning them from data. The main scientific objective of this project is to create new informative and compact representations of deformable objects that incorporate both analytical and learning-based approaches and encode geometric, topological, and physical information about the robot, the object, and the environment. We will do this in the context of challenging multimodal, bimanual object interaction tasks. The focus will be on physical interaction with deformable objects using multimodal feedback. To meet these objectives, we will use theoretical and computational methods together with rigorous experimental evaluation to model skilled sensorimotor behavior in bimanual robot systems.
Summary
All day long, our fingers touch, grasp and move objects in various media such as air, water, oil. We do this almost effortlessly - it feels like we do not spend time planning and reflecting over what our hands and fingers do or how the continuous integration of various sensory modalities such as vision, touch, proprioception, hearing help us to outperform any other biological system in the variety of the interaction tasks that we can execute. Largely overlooked, and perhaps most fascinating is the ease with which we perform these interactions resulting in a belief that these are also easy to accomplish in artificial systems such as robots. However, there are still no robots that can easily hand-wash dishes, button a shirt or peel a potato. Our claim is that this is fundamentally a problem of appropriate representation or parameterization. When interacting with objects, the robot needs to consider geometric, topological, and physical properties of objects. This can be done either explicitly, by modeling and representing these properties, or implicitly, by learning them from data. The main scientific objective of this project is to create new informative and compact representations of deformable objects that incorporate both analytical and learning-based approaches and encode geometric, topological, and physical information about the robot, the object, and the environment. We will do this in the context of challenging multimodal, bimanual object interaction tasks. The focus will be on physical interaction with deformable objects using multimodal feedback. To meet these objectives, we will use theoretical and computational methods together with rigorous experimental evaluation to model skilled sensorimotor behavior in bimanual robot systems.
Max ERC Funding
2 424 186 €
Duration
Start date: 2020-09-01, End date: 2025-08-31
Project acronym BONE SCAN
Project Traces in the bones: reconstructing the lost soft anatomy of the earliest vertebrates through ultra-high resolution synchrotron scanning
Researcher (PI) Per Erik Ahlberg
Host Institution (HI) UPPSALA UNIVERSITET
Country Sweden
Call Details Advanced Grant (AdG), LS8, ERC-2008-AdG
Summary Early vertebrate evolution involved a series of drastic structural reorganisations as new features were added and elaborated. The fossil record illuminates this evolutionary history more directly than inferences from the diversity of living forms, but the fossils usually consist only of bones whereas many of the most important and interesting changes occurred in the soft anatomy. Traditional approaches to reconstructing the musculature and other soft tissues of fossil vertebrates rely on subjective tools, like the visual identification of rough bone textures thought to indicate muscle attachments, and generally leave a lot to be desired. Here I propose a wholly novel and radically more objective approach to the identification of soft-tissue contacts, using holotomographic synchrotron CT at sub-micron resolutions to identify these contacts by the three-dimensional micro-architecture of the bone. A pilot study has already shown that such scans (performed at the ESRF synchrotron facility in Grenoble) are capable of imaging key features such as arrested growth surfaces and probable Sharpey s fibres in 380 million year old fossils. We will undertake a systematic review of the three-dimensional bone micro-architectures associated with different soft-tissue contacts in living vertebrates, and the use this as a key to reconstruct the soft-tissue contacts on fossil bones with unprecedented accuracy. This will permit us to produce far more reliable reconstructions of the soft anatomy than has hitherto been possible. Our findings will inform other areas of palaentology, particularly functional morphology, and will also be of great importance to evolutionary developmental biology.
Summary
Early vertebrate evolution involved a series of drastic structural reorganisations as new features were added and elaborated. The fossil record illuminates this evolutionary history more directly than inferences from the diversity of living forms, but the fossils usually consist only of bones whereas many of the most important and interesting changes occurred in the soft anatomy. Traditional approaches to reconstructing the musculature and other soft tissues of fossil vertebrates rely on subjective tools, like the visual identification of rough bone textures thought to indicate muscle attachments, and generally leave a lot to be desired. Here I propose a wholly novel and radically more objective approach to the identification of soft-tissue contacts, using holotomographic synchrotron CT at sub-micron resolutions to identify these contacts by the three-dimensional micro-architecture of the bone. A pilot study has already shown that such scans (performed at the ESRF synchrotron facility in Grenoble) are capable of imaging key features such as arrested growth surfaces and probable Sharpey s fibres in 380 million year old fossils. We will undertake a systematic review of the three-dimensional bone micro-architectures associated with different soft-tissue contacts in living vertebrates, and the use this as a key to reconstruct the soft-tissue contacts on fossil bones with unprecedented accuracy. This will permit us to produce far more reliable reconstructions of the soft anatomy than has hitherto been possible. Our findings will inform other areas of palaentology, particularly functional morphology, and will also be of great importance to evolutionary developmental biology.
Max ERC Funding
1 046 782 €
Duration
Start date: 2009-04-01, End date: 2014-03-31
Project acronym BOTMED
Project Microrobotics and Nanomedicine
Researcher (PI) Bradley James Nelson
Host Institution (HI) EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Country Switzerland
Call Details Advanced Grant (AdG), PE7, ERC-2010-AdG_20100224
Summary The introduction of minimally invasive surgery in the 1980’s created a paradigm shift in surgical procedures. Health care is now in a position to make a more dramatic leap by integrating newly developed wireless microrobotic technologies with nanomedicine to perform precisely targeted, localized endoluminal techniques. Devices capable of entering the human body through natural orifices or small incisions to deliver drugs, perform diagnostic procedures, and excise and repair tissue will be used. These new procedures will result in less trauma to the patient and faster recovery times, and will enable new therapies that have not yet been conceived. In order to realize this, many new technologies must be developed and synergistically integrated, and medical therapies for which the technology will prove successful must be aggressively pursued.
This proposed project will result in the realization of animal trials in which wireless microrobotic devices will be used to investigate a variety of extremely delicate ophthalmic therapies. The therapies to be pursued include the delivery of tissue plasminogen activator (t-PA) to blocked retinal veins, the peeling of epiretinal membranes from the retina, and the development of diagnostic procedures based on mapping oxygen concentration at the vitreous-retina interface. With successful animal trials, a path to human trials and commercialization will follow. Clearly, many systems in the body have the potential to benefit from the endoluminal technologies that this project considers, including the digestive system, the circulatory system, the urinary system, the central nervous system, the respiratory system, the female reproductive system and even the fetus. Microrobotic retinal therapies will greatly illuminate the potential that the integration of microrobotics and nanomedicine holds for society, and greatly accelerate this trend in Europe.
Summary
The introduction of minimally invasive surgery in the 1980’s created a paradigm shift in surgical procedures. Health care is now in a position to make a more dramatic leap by integrating newly developed wireless microrobotic technologies with nanomedicine to perform precisely targeted, localized endoluminal techniques. Devices capable of entering the human body through natural orifices or small incisions to deliver drugs, perform diagnostic procedures, and excise and repair tissue will be used. These new procedures will result in less trauma to the patient and faster recovery times, and will enable new therapies that have not yet been conceived. In order to realize this, many new technologies must be developed and synergistically integrated, and medical therapies for which the technology will prove successful must be aggressively pursued.
This proposed project will result in the realization of animal trials in which wireless microrobotic devices will be used to investigate a variety of extremely delicate ophthalmic therapies. The therapies to be pursued include the delivery of tissue plasminogen activator (t-PA) to blocked retinal veins, the peeling of epiretinal membranes from the retina, and the development of diagnostic procedures based on mapping oxygen concentration at the vitreous-retina interface. With successful animal trials, a path to human trials and commercialization will follow. Clearly, many systems in the body have the potential to benefit from the endoluminal technologies that this project considers, including the digestive system, the circulatory system, the urinary system, the central nervous system, the respiratory system, the female reproductive system and even the fetus. Microrobotic retinal therapies will greatly illuminate the potential that the integration of microrobotics and nanomedicine holds for society, and greatly accelerate this trend in Europe.
Max ERC Funding
2 498 044 €
Duration
Start date: 2011-04-01, End date: 2016-03-31
Project acronym CoCi
Project Co-Evolving City Life
Researcher (PI) Dirk HELBING
Host Institution (HI) EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Country Switzerland
Call Details Advanced Grant (AdG), SH2, ERC-2018-ADG
Summary How could networks of innovative cities contribute to the solution of humanity’s existential problems? Given the on-going digital revolution and our present-day sustainability challenges, we have to reinvent the way cities are operated. We propose that the requirement of organizing cities in a more resilient way implies the need for more decentralized solutions, based on digitally assisted self-organization, and that this concept is also compatible with sustainability requirements and stronger democratic participation. The CoCi proposal will investigate, whether such a decentralized, participatory approach could compete with a fully centralized approach in terms of efficiency and sustainability, or perform even better than that. This requires in particular to figure out, how distributed co-creation processes can be coordinated and lifted to a professional level in a scalable way. The main questions of the CoCi proposal are: How could more participatory smart cities work, and how can they meet the requirements of being more efficient, sustainable and resilient? What are their risks and benefits compared with centralized approaches? How could digital societies fitting our culture, for example, based on values such as freedom, equality and solidarity (liberté, égalité, fraternité) look like, and what performance can be expected from them? The CoCi proposal brings together two research directions: first, the automation of mobility solutions based on the Internet of Things and Machine Learning approaches, as they have been pursued within the “smart cities” paradigm and, second, novel collaborative approaches as they have been recently discussed under labels such as participatory resilience, digital democracy, City Olympics, open source urbanism, and the “socio-ecological finance system”.
Summary
How could networks of innovative cities contribute to the solution of humanity’s existential problems? Given the on-going digital revolution and our present-day sustainability challenges, we have to reinvent the way cities are operated. We propose that the requirement of organizing cities in a more resilient way implies the need for more decentralized solutions, based on digitally assisted self-organization, and that this concept is also compatible with sustainability requirements and stronger democratic participation. The CoCi proposal will investigate, whether such a decentralized, participatory approach could compete with a fully centralized approach in terms of efficiency and sustainability, or perform even better than that. This requires in particular to figure out, how distributed co-creation processes can be coordinated and lifted to a professional level in a scalable way. The main questions of the CoCi proposal are: How could more participatory smart cities work, and how can they meet the requirements of being more efficient, sustainable and resilient? What are their risks and benefits compared with centralized approaches? How could digital societies fitting our culture, for example, based on values such as freedom, equality and solidarity (liberté, égalité, fraternité) look like, and what performance can be expected from them? The CoCi proposal brings together two research directions: first, the automation of mobility solutions based on the Internet of Things and Machine Learning approaches, as they have been pursued within the “smart cities” paradigm and, second, novel collaborative approaches as they have been recently discussed under labels such as participatory resilience, digital democracy, City Olympics, open source urbanism, and the “socio-ecological finance system”.
Max ERC Funding
2 499 500 €
Duration
Start date: 2020-10-01, End date: 2025-09-30
Project acronym CyberGenetics
Project Cybergenetics: Theory and Design Tools for Biomolecular Control Systems
Researcher (PI) Mustafa KHAMMASH
Host Institution (HI) EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Country Switzerland
Call Details Advanced Grant (AdG), PE7, ERC-2016-ADG
Summary We propose to develop a new theory and design tools for the estimation and real-time control of living cells. The control systems designed using these tools will precisely and robustly steer the dynamic behavior of living cells in real time to achieve desired objectives. Cells would be controlled either collectively at the population level, or individually as single cells. The control systems achieving this regulation will be realized either on a digital computer that is interfaced with living cells, or using de novo genetic circuits that are introduced into the cells where they are designed to function as molecular control systems. Our methods will explicitly confront the numerous challenges brought about by the special environment of the cell including nonlinearity, stochasticity, cell-to-cell variability, metabolic burden, etc. The theory and methods developed in this project will thus enable the systematic, rational, and effective feedback control of living cells at the gene level, and will lay the foundation for a new corresponding body of knowledge which we call ``Cybergenetics''. It will also open new research directions in the areas of control theory and estimation.
We also propose to design three cybergenetic control systems, each addressing an important application in biotechnology or therapeutics. In the first, the controller will use light and nutrient supply to precisely regulate gene expression and cell growth in E. coli to achieve high protein and low biomass production rates. The second involves multiple feedback controllers regulating in parallel a large number of single stem cells, and leading to their differentiation to desired fates, e.g. beta cells, with potential for therapeutic applications. Finally, we will engineer into living cells dynamic molecular control systems. Such controllers can be used to monitor physiological variables and secrete biological effectors in a feedback fashion for the treatment of diseases like Type 1 diabetes.
Summary
We propose to develop a new theory and design tools for the estimation and real-time control of living cells. The control systems designed using these tools will precisely and robustly steer the dynamic behavior of living cells in real time to achieve desired objectives. Cells would be controlled either collectively at the population level, or individually as single cells. The control systems achieving this regulation will be realized either on a digital computer that is interfaced with living cells, or using de novo genetic circuits that are introduced into the cells where they are designed to function as molecular control systems. Our methods will explicitly confront the numerous challenges brought about by the special environment of the cell including nonlinearity, stochasticity, cell-to-cell variability, metabolic burden, etc. The theory and methods developed in this project will thus enable the systematic, rational, and effective feedback control of living cells at the gene level, and will lay the foundation for a new corresponding body of knowledge which we call ``Cybergenetics''. It will also open new research directions in the areas of control theory and estimation.
We also propose to design three cybergenetic control systems, each addressing an important application in biotechnology or therapeutics. In the first, the controller will use light and nutrient supply to precisely regulate gene expression and cell growth in E. coli to achieve high protein and low biomass production rates. The second involves multiple feedback controllers regulating in parallel a large number of single stem cells, and leading to their differentiation to desired fates, e.g. beta cells, with potential for therapeutic applications. Finally, we will engineer into living cells dynamic molecular control systems. Such controllers can be used to monitor physiological variables and secrete biological effectors in a feedback fashion for the treatment of diseases like Type 1 diabetes.
Max ERC Funding
2 499 887 €
Duration
Start date: 2017-08-01, End date: 2022-07-31
Project acronym DALDECS
Project Development and Application of Laser Diagnostic Techniques for Combustion Studies
Researcher (PI) Lars Eric Marcus Alden
Host Institution (HI) MAX IV Laboratory, Lund University
Country Sweden
Call Details Advanced Grant (AdG), PE8, ERC-2009-AdG
Summary This project is directed towards development of new laser diagnostic techniques and a deepened physical understanding of more established techniques, aiming at new insights in phenomena related to combustion processes. These non-intrusive techniques with high resolution in space and time, will be used for measurements of key parameters, species concentrations and temperatures. The techniques to be used are; Non-linear optical techniques, mainly Polarization spectroscopy, PS. PS will mainly be developed for sensitive detection with high spatial resolution of "new" species in the IR region, e.g. individual hydrocarbons, toxic species as well as alkali metal compounds. Multiplex measurements of these species and temperature will be developed as well as 2D visualization. Quantitative measurements with high precision and accuracy; Laser induced fluorescence and Rayleigh/Raman scattering will be developed for quantitative measurements of species concentration and 2D temperatures. Also a new technique will be developed for single ended experiments based on picosecond LIDAR. Advanced imaging techniques; New high speed (10-100 kHz) visualization techniques as well as 3D and even 4D visualization will be developed. In order to properly visualize dense sprays we will develop Ballistic Imaging as well as a new technique based on structured illumination of the area of interest for suppression of multiple scattering which normally cause blurring effects. All techniques developed above will be used for key studies of phenomena related to various combustion phenomena; turbulent combustion, multiphase conversion processes, e.g. spray combustion and gasification/pyrolysis of solid bio fuels. The techniques will also be applied for development and physical understanding of how combustion could be influenced by plasma/electrical assistance. Finally, the techniques will be prepared for applications in industrial combustion apparatus, e.g. furnaces, gasturbines and IC engines
Summary
This project is directed towards development of new laser diagnostic techniques and a deepened physical understanding of more established techniques, aiming at new insights in phenomena related to combustion processes. These non-intrusive techniques with high resolution in space and time, will be used for measurements of key parameters, species concentrations and temperatures. The techniques to be used are; Non-linear optical techniques, mainly Polarization spectroscopy, PS. PS will mainly be developed for sensitive detection with high spatial resolution of "new" species in the IR region, e.g. individual hydrocarbons, toxic species as well as alkali metal compounds. Multiplex measurements of these species and temperature will be developed as well as 2D visualization. Quantitative measurements with high precision and accuracy; Laser induced fluorescence and Rayleigh/Raman scattering will be developed for quantitative measurements of species concentration and 2D temperatures. Also a new technique will be developed for single ended experiments based on picosecond LIDAR. Advanced imaging techniques; New high speed (10-100 kHz) visualization techniques as well as 3D and even 4D visualization will be developed. In order to properly visualize dense sprays we will develop Ballistic Imaging as well as a new technique based on structured illumination of the area of interest for suppression of multiple scattering which normally cause blurring effects. All techniques developed above will be used for key studies of phenomena related to various combustion phenomena; turbulent combustion, multiphase conversion processes, e.g. spray combustion and gasification/pyrolysis of solid bio fuels. The techniques will also be applied for development and physical understanding of how combustion could be influenced by plasma/electrical assistance. Finally, the techniques will be prepared for applications in industrial combustion apparatus, e.g. furnaces, gasturbines and IC engines
Max ERC Funding
2 466 000 €
Duration
Start date: 2010-02-01, End date: 2015-01-31