New approach in amphibian biology: harmless viruses help to study the nervous system of frogs
27 November 2024
Amphibians hold a significant place in evolution, representing the transition from aquatic to terrestrial lifestyles. They are crucial for understanding the brain and spinal cord of tetrapods-animals with four limbs, including humans. A group of scientists led by a team at the Institute of Science and Technology Austria (ISTA) now shows how harmless viruses can be used to illuminate the development of the frog nervous system. The results have now been published in Developmental Cell. 
New approach in amphibian biology: harmless viruses help to study the nervous system of frogs

Virus. When you hear the word, you probably shudder. But not all viruses are bad or cause disease. Some are even used for therapeutic applications or vaccination. In basic research, they are often employed to infect certain cells, genetically modify them, or visualize neurons in the organism’s central nervous system (CNS)—the command center made up of the brain, spinal cord, and nerves.

The highlighting process now finally works in amphibians. This has been shown in a new study by an international EDGE consortium jointly led by the Sweeney Lab at the Institute of Science and Technology Austria (ISTA) and the Tosches Lab at Columbia University. The researchers established a new technique that uses adeno-associated viruses (AAVs) to track a frog's nervous system throughout its metamorphosis—a developmental transition from the early tadpole stages to its adult form. A breakthrough that can help usher amphibian neurobiology into a new era.


Swimming vs. walking


David Vijatovic and Lora Sweeney enter a laboratory full of water tanks. Vijatovic taps on one of them. Inside, a small mottled greenish-brown African clawed frog (Xenopus laevis) appears. Its limbs are prominent, gracefully maneuvering and gripping its surroundings. In another tank, tadpoles are swirling around using simple swimming motions. It is remarkable to think that one transforms into the other.

‘Frogs undergo metamorphosis,’ Sweeney says, ‘making them a great model organism for studying the transition between two movement modes—swimming and walking.’ A frog’s development spans over 12 to 16 weeks, giving scientists time to study each stage. During these weeks, a frog embryo develops to a young tadpole, a tadpole with two legs, and a young froglet with four legs before reaching the adult stage. ‘By looking at the several stages of development, we can investigate these locomotive behaviors and the underlying changes in the nervous system,’ Vijatovic adds.


Just like an electrical circuit: how frogs are wired


An organism’s nervous system is referred to as the neural circuit because it resembles an electrical circuit. ‘Nerve cells (neurons) are connected to other neurons, transmitting electrical information. How we behave, what we sense, and how we interact with the world are the product of the way our neurons communicate with each other within these circuits,’ explains Sweeney. The critical piece is how the circuit is wired. We know that neurons are connected but which neuron connects to which? Which other cells does a single cell talk to, and what messages does it convey?

To know more about this wiring, researchers have been using viruses, proven to be a powerful tool. Adeno-associated viruses (AAVs) are ideal in that regard. They are non-pathogenic while being able to infect a wide range of cell types, including neurons. AAVs can be modified to glow in bright green fluorescent colours under the microscope as they travel along neurons, whether in retrograde (backward, from the synapse toward the cell body) or anterograde (forward, from the cell body toward the synapse). In other words, AAVs can be used to illuminate the neural circuit from the broadcasting end to the receiving end or vice-versa.  

‘This is a common technique used in neuroscience, especially in well-studied organisms like mice. For amphibians, it was thought that it could not be done,’ says Vijatovic. That was the general belief until now.


The power of scientific collaboration 


To make AAV labeling work in amphibians, Sweeney and Vijatovic joined forces with an international team of scientists from Maria Tosches’ group at Columbia University, where the study’s other two co-first authors Eliza Jaeger and Astrid Deryckere are based. The consortium also included researchers from Tel Aviv University, the University of Utah, the Scripps Research Institute, and the California Institute of Technology. The researchers put their heads together, drew expertise from each other, visited conferences, had countless Zoom calls, and came up with different perspectives and ideas. ‘When you start researching an organism that is not yet well understood, it is great to have a community where you can share information,’ says Sweeney.  

They screened existing AAVs to find what was suitable for amphibians and optimized the infecting strategy eventually developing a “how-to guide” for frogs and newts. Vijatovic summarizes his PhD journey, ‘We started with young tadpoles, made our way to older tadpoles, and finally moved to juvenile and then adult frogs as well as adult newts. We tailored the tool to each life stage.’


Comparing frogs to humans: what this research says about us 


With this new technique, the scientists managed to apply AAVs for tracing neuron connections in amphibians. This will help them find out more about how the amphibian brain compares to that of mammals. Besides that, the new approach also opens doors to further analysing neuronal development. With some of the screened AAV variants, the researchers can label progenitor cells at a specific point in time during the circuit’s development and follow them to see what neurons they become. ‘This way, we can resolve the whole circuit by its development, see how it changes over time, and how the whole nervous system is built,’ Sweeney says.  

Although amphibians and mammals last shared a common ancestor about 360 million years ago, they share common traits. ‘By comparing the details of a frog’s nervous system to a human's, we can see what we don’t have and what we have,’ Sweeney continues. This knowledge can help us understand how the human nervous system became specialized over time. ‘The better we understand the basic building blocks of the nervous system, the more we understand how we can replace them during disease and injury.’ 


Publication


E.C.B. Jaeger, D. Vijatovic, A. Deryckere, N. Zorin, A. L. Nguyen, G. Ivanian, J. Woych, R. C. Arnold, A. Ortega Gurrola, A. Shvartsman, F. Barbieri, F. A. Toma, H. T. Cline, T.F. Shay, D. B. Kelley, A. Yamaguchi, M. Shein-Idelson, M. A. Tosches and L. B. Sweeney. 2024. Adeno-associated viral tools to trace neural development and connectivity across amphibians. Developmental Cell. DOI: 10.1016/j.devcel.2024.10.025


Funding information


The ISTA part of the project was supported by funding from the National Science Foundation EDGE Award IOS 2110086, the FTI Strategy Lower Austria Dissertation FT121-D-046; the Horizon Europe ERC Starting Grant 101041551, the Special Research Programme (SFB) of the Austrian Science Fund (FWF) project F7814-B & the NIH grant R35GM146973.


Information on animal studies


In order to better understand fundamental processes, for example, in the fields of neuroscience, immunology, or genetics, the use of animals in research is indispensable. No other methods, such as in silico models, can serve as alternative. The animals are raised, kept, and treated according to the strict regulations of Austrian law. All animal procedures are approved by the Federal Ministry of Education, Science and Research.


About ISTA
 

The Institute of Science and Technology Austria (ISTA) is a PhD-granting research institution located in Klosterneuburg, 18 km from the center of Vienna, Austria. ISTA employs professors on a tenure-track model, post-doctoral researchers and PhD students. The Graduate School of ISTA offers fully funded PhD positions to highly qualified candidates with a Bachelor’s or Master’s degree in biology, mathematics, computer science, physics, chemistry, and related areas. While dedicated to the principle of curiosity-driven research, ISTA aims to deliver scientific findings to society through technological transfer and science education. President of the Institute is Martin Hetzer, a renowned molecular biologist and former Senior Vice President at The Salk Institute for Biological Studies in California, USA

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Project information

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swim2limb
Development and Evolution of Tetrapod Motor Circuits 

Researcher: Lora Sweeney 

Host institution: The Institute of Science and Technology Austria (ISTA) 

Call details: ERC-2021-STG 101041551, Life Science, LS5 – Neuroscience & Disorders of the Nervous Systems

ERC funding: €1 500 000

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Press contacts

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Andreas Rothe
The Institute of Science and Technology Austria (ISTA)  
T: +43 664 8832 6510  

Marcin Mońko
Head of Media and Content sector
T: +32 2 296 66 44